A greener approach to the study of the green: sustainable liquid chromatography applied to the metabolite profile investigation of Brazilian medicinal plants

• Grant number: 12/15844-7
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: November 01, 2012
• End date: October 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Alberto Jose Cavalheiro
• Grantee: Cristiano Soleo de Funari
• Supervisor: Emily F. Hilder
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University of Tasmania (UTAS), Australia
• Associated to the scholarship: 10/18840-7 - Green Liquid Chromatography (water, ethanol and temperature)applied to the study of medicinal plants, BP.PD

Abstract

Despite the eco-friendly claim usually employed to promote products exploiting biodiversity, harmful solvents are often employed in both phytochemical research and the quality control analyses of such products. Gas Chromatography and Supercritical Fluid Chromatography have inherent characteristics of Green technology, but Liquid Chromatography can be applied in a sustainable way by the reduction, substitution or elimination of harmful solvents. This project focus on the development of sustainable high-performance liquid chromatography methods for the analyses of Brazilian plants by employing pure water or water-ethanol mixtures as mobile phase (thus, eliminating methanol and acetonitrile or substitute them for the non toxic, biodegradable and largely produced in Brazil ethanol). Despite the high viscosity of ethanol/water mixtures (the claimed technical obstacle to the popularization of its application when fine particles packed columns and convetional High-Performance Liquid Chromatography machines are used, the most avilable worldwide), and of the low eluotropic force of pure water on revesed-phase chromatography, termostable monolithic columns and high-temperature separations will be exploited to circumvent the challenges and achieve the optimal use of these mobile phases in our research. Methods will be developed from an original and innovative response which not only maximizes the number of peaks and their distribution across the chromatogram but also attempts to minimize the time and consumption of resources such as energy and solvents. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRISTIANO SOLEO FUNARI; IAN CASTRO-GAMBOA; ALBERTO JOSÉ CAVALHEIRO; VANDERLAN DA SILVA BOLZANI. Metabolômica, uma abordagem otimizada para exploração da biodiversidade brasileira: estado da arte, perspectivas e desafios. Química Nova, v. 36, n. 10, p. 1605-1609, 2013-00-00. (12/15844-7)

ARDILA, JORGE ARMANDO; FUNARI, CRISTIANO SOLEO; ANDRADE, ANDRE MARQUES; CAVALHEIRO, ALBERTO JOSE; CARNEIRO, RENATO LAJARIM. Cluster analysis of commercial samples of Bauhinia spp. using HPLC-UV/PDA and MCR-ALS/PCA without peak alignment procedure. Phytochemical Analysis, v. 26, n. 5, p. 367-373, SEP-OCT 2015. (13/07600-3, 12/15844-7, 10/16520-5)

FUNARI, CRISTIANO SOLEO; CARNEIRO, RENATO LAJARIM; ANDRADE, ANDRE MARQUES; HILDER, EMILY FRANCES; CAVALHEIRO, ALBERTO JOSE. Green chromatographic fingerprinting: An environmentally friendly approach for the development of separation methods for fingerprinting complex matrices. JOURNAL OF SEPARATION SCIENCE, v. 37, n. 1-2, p. 37-44, JAN 2014. (12/15844-7, 10/16520-5)

ARDILA, JORGE ARMANDO; FUNARI, CRISTIANO SOLEO; ANDRADE, ANDRE MARQUES; CAVALHEIRO, ALBERTO JOSE; CARNEIRO, RENATO LAJARIM. Cluster analysis of commercial samples of Bauhinia spp. using HPLC-UV/PDA and MCR-ALS/PCA without peak alignment procedure. Phytochemical Analysis, v. 26, n. 5, p. 7-pg., 2015-09-01. (12/15844-7, 10/16520-5, 13/07600-3)

FUNARI, CRISTIANO SOLEO; CARNEIRO, RENATO LAJARIM; CREESE, MARI EGENESS; LEME, GABRIEL MAZZI; CAYALHEIRO, ALBERTO JOSE; HILDER, EMILY FRANCES. On Track for a Truly Green Propolis-Fingerprinting Propolis Samples from Seven Countries by Means of a Fully Green Approach. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 4, n. 12, p. 7110-7117, DEC 2016. (10/18840-7, 13/07600-3, 12/15844-7, 10/16520-5)

FUNARI, CRISTIANO SOLEO; CARNEIRO, RENATO LAJARIM; KHANDAGALE, MANISH M.; CAVALHEIRO, ALBERTO JOSE; HILDER, EMILY F.. Acetone as a greener alternative to acetonitrile in liquid chromatographic fingerprinting. JOURNAL OF SEPARATION SCIENCE, v. 38, n. 9, p. 1458-1465, MAY 2015. (13/07600-3, 12/15844-7)