Adjuvant effect of killed-Propionibacterium acnes on dendritic cells vaccine for prophilaxis and therapy of murine melanoma

• Grant number: 12/09885-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): September 01, 2012
• Effective date (End): February 28, 2017
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Ieda Maria Longo Maugéri
• Grantee: Mayari Eika Ishimura
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The use of adjuvants to enhance and/or modulate protective and effective immune response is very important, once that it effectiveness has been observed on immunotherapy and immunoprophylaxis. Among the adjuvants substances studied stand out biologic adjuvants, like Propionibacterium acnes (P. acnes). The focus of the our lab is study the immunomodulatory effects of heat-killed P. acnes suspension and its purified soluble polysaccharide extracted from P. acnes cell wall (PS) on innate and adaptive immune response in different experimental models. It is know that P. acnes increases phagocytic and tumoricidal macrophage activity, potentiates the lipopolysaccharide (LPS) induced endotoxic shock, enhances resistance to infections, induces pro-inflammatory cytokines and increases humoral response to different antigens. The PS is an important bacterial component for inducing biologic effects similar to that obtained on animals treated with heat-killed P. acnes suspension. This bacterium and its PS also act on dendritic cells precursors, promoting recruitment, activation and maturation of these cells. The protective and effective immune response is driven by dendritic cell in the moment of antigen presentation. This process depends on cytokines produced by dendritic cells, as well as their surface molecules expression and activation ways, which can be modulated by P. acnes. Autologous dendritic cells, manipulated in vitro with tumoral extract employed on treatment of neoplasia has been subject of the different clinical and experimental studies. In this work, we propose to evaluate how dendritic cells matured in culture with P. acnes or PS, pulsed in vitro with tumoral antigens, act on specific anti-tumoral immune response, using the murine melanoma model. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ISHIMURA, MAYARI E.; TEIXEIRA, DANIELA; SILVEIRA, GABRIELA DA P.; GAMBERO, MONICA; GAMA, GABRIEL A. C.; PIMENTA, BRUNA S. O.; RODRIGUES, ELAINE G.; LONGO-MAUGERI, IEDA M.. Killed Propionibacterium acnes enhances immunogenicity and tumor growth control of a dendritic-tumor cell hybrid vaccine in a murine melanoma model. PLoS One, v. 13, n. 10, OCT 9 2018. (12/09885-2)