Pluripotency analysis of stem cells-like isolated from fetal tissue

• Grant number: 12/14674-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2012
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Irina Kerkis
• Grantee: Damiana Pedro de Oliveira
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 10/51051-6 - Adult stem cells versus induced pluripotent stem cells, AP.TEM

Abstract

Pluripotent stem cells (PSC) have ability of self-renewal and differentiate into all cell types of the embryo. The pluripotency can be demonstrated by several experiments in vitro and in vivo such as expression of pluripotent markers (oct3 / 4 and NANOG), differentiation capacity in all cell types of the three germ layers and chimera formation. PSC are isolated in vitro from early stages of development (inner cell mass and epiblast). In recent experiments, we isolated pluripotent stem cells-similes (PSCs) from fetal tissue. These results suggest that is possible isolation of PSC from later stages of development. Stem cells (SC), isolated from fetal tissue has properties more primitive than the adults stem cells with a high potential for growth in vitro and expression of pluripotency markers. The isolation of PSCs in later stages of development to date has not been investigated. Based on these recent findings, this project aims to evaluate the expression of pluripotency markers (oct3 / 4 and NANOG) in PSCs isolated from fetal brain in order to confirm the pluripotency of these cells. For this purpose for the isolation of SPCs in vitro will be used fetal mice with 12, 15 and 18 days of development. The expression is evaluated through technique of RT-PCR and immunostaining. The study will provide important information about conditions of isolation of PSCs and mechanism for maintenance of pluripotency and differentiation in vitro.(AU)