Evaluation of migration and invasion in cell lines of breast cancer in response to genistein treatment

• Grant number: 12/15596-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Debora Aparecida Pires de Campos Zuccari
• Grantee: Paula de Almeida Marques
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Breast cancer is the second most common cancer among women and the intense tumor growth and metastasis are still providing high levels of mortality from the disease. The invasiveness, cell migration and tumor implantation are peculiarities of metastatic cells that allow the spread of disease and consequent worse prognosis. Genistein, found predominantly isoflavones in soy products, has proved a potent inhibitor of carcinogenesis in animal models. This is due to ability of isoflavones to negatively regulate metastasis. The objective of this study is to evaluate in vitro the effects of treatment with genistein in breast cancer metastasis. Assays migration and invasion of metastatic lines MDA-MB-231 and non-metastatic MCF-7 are performed in Boyden chamber, a method for evaluating the metastatic potential of cancer cells in vitro. The data obtained in this study may provide clues to the use of genistein as a therapeutic agent in the treatment of breast cancer, preventing the formation of metastases. (AU)