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Suitability and effect of a controlled drug release system in presence of patogenic biofilm

Grant number: 12/15556-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2012
Effective date (End): November 30, 2014
Field of knowledge:Health Sciences - Pharmacy - Pharmaceutical Technology
Principal Investigator:Carolina Patrícia Aires
Grantee:Ana Carolina dos Santos Ré
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The biofilm is the etiological factor of most oral diseases as caries, gingivitis and periodontitis. In this regard, sustained release systems of drugs have been developed to the oral cavity in order to become less virulent biofilm. However, few studies have proposed models that simulate the conditions found in the mouth, such as bacteria organized in a biofilm. The objective of this study is to obtain a semi-solid-releasing drug that is feasible for the conditions considering the presence of oral bacteria, its extracellular products and the dynamics of staying in a liquid medium replacement daily. Thus, a system in situ gelation based on glyceryl monooleate is prepared and a metronidazole-containing formulation to be used as a standard tests aimed at obtaining a system able to provide stable preservation against biofilm of Streptococcus mutans. After the trial period for biofilm growth, it will be collected and homogenized by sonication. Viability is monitored by bacterial count of bacteria on BHI agar and the amount of drug remaining in the biofilm will be analyzed by HPLC. The formulation will be evaluated in relation to its mass, crystalline mesophase and the active residual. For comparison between groups, will initially be analyzed the homogeneity and variability of the results. The significance level of 5% is accepted.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RE, A. C. S.; FERREIRA, M. P.; FREITAS, O.; AIRES, C. P. Antimicrobial effect of a local release system containing metronidazole against a Porphyromonas gingivalis biofilm. Pharmazie, v. 74, n. 11, p. 665-666, NOV 2019. Web of Science Citations: 0.
RE, A. C. S.; FERREIRA, M. P.; FREITAS, O.; AIRES, C. P. Local antibiotic delivery in periodontitis: drug release and its effect on supragingival biofilms. BIOFOULING, v. 32, n. 9, p. 1061-1066, 2016. Web of Science Citations: 4.

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