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Identification of metastasis suppressor genes

Grant number: 12/03771-5
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2012
Effective date (End): August 31, 2013
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal Investigator:Maria Cristina Cintra Gomes Marcondes
Grantee:Rebeka Tomasin
Supervisor abroad: Robin Lesley Anderson
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Local de pesquisa : University of Melbourne, Australia  
Associated to the scholarship:10/00714-5 - "modulatory Effects of Aloe vera ánd honey ín tumour growth", BP.DR


Metastases are the major cause of death among cancer patients, especially due to therapies for these patients are extremely limited. By identifying the genes that control metastasis, it will be possible to predict the patients whose disease is likely to spread. Furthermore, once the genes that are critical to metastasis of breast cancer are known, an opportunity exists to develop therapies that target these genes and thereby provide these patients with more effective treatments. The aim of this work is to identify genes whose reduction leads to metastasis to secondary sites through a shRNA library screening. The poorly metastatic mammary tumor line 66cl4 will be infected with a lentiviral mouse shRNA library and the successfully infected cells will be inoculated into Balb/c mice. The metastatic nodules in these mice will be analyzed to identify the gene that has been knocked down. Once identified, in vivo and in vitro experiments will be conducted to validate the targeted gene as a metastasis suppressor gene and also elucidating the gene's function, respectively. The expression of the gene in human breast cancer will also be assessed to determine if it is a prognostic factor for outcome in breast cancer patients. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTIN, ANA CAROLINA B. M.; FUZER, ANGELINA M.; BECCENERI, AMANDA B.; DA SILVA, JAMES ALMADA; TOMASIN, REBEKA; DENOYER, DELPHINE; KIM, SOO-HYUN; MCINTYRE, KATHERINE A.; PEARSON, HELEN B.; YEO, BELINDA; NAGPAL, AADYA; LING, XIAWEI; SELISTRE-DE-ARAUJO, HELOISA S.; VIEIRA, PAULO CEZAR; COMINETTI, MARCIA R.; POULIOT, NORMAND. [10]-gingerol induces apoptosis and inhibits metastatic dissemination of triple negative breast cancer in vivo. ONCOTARGET, v. 8, n. 42, p. 72260-72271, SEP 22 2017. Web of Science Citations: 14.

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