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Chemical microstructure analisys and sealing ability of MTA-based root canal sealers under different humidity environmental conditions

Grant number: 12/11035-7
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 05, 2012
Effective date (End): July 04, 2013
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:Mario Tanomaru Filho
Grantee:Raqueli Viapiana
Supervisor abroad: Josette Camilleri
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Research place: University of Malta (UoM), Malta  
Associated to the scholarship:10/17976-2 - Physico-chemical and mecanichal evaluation of mineral trioxide aggregate based root canal sealers in comparison to AH Plus and Sealapex., BP.DR

Abstract

The aim of this study is to evaluate the chemical microstructure and sealing ability of four experimental MTA-based root canal sealers with nano and micro particles of zirconium oxide or niobium oxide in comparison to MTA Fillapex, Sealapex and AH Plus. Material characterization will be performed using a combination of scanning electron microscopy (SEM) with X-ray energy dispersive (EDX) analyses, X-ray diffraction (XRD) and infrared spectroscopy (FTIR-ATR) analyses. Standard specimens will be confectioned (n=5) and the analysis will be applied on cured sealers submitted to different humidity conditions namely at 100% humidity or immersed in either water or Hank's balanced salt solution (HBSS) for 1 and 28 days at 37 æC. To evaluate the sealing ability of the sealers, seventy four single-canal human teeth will be decoronated and biomechanically prepared to be obturated with the sealers and guta-percha. A needle inserted across an acrylic platform will be introduced in the canal orifice and tooth assembly will be placed in 10 mL of HBSS keeping the free end of the needle unimersed and the coronal end of the tooth not in contact with biological fluids. The samples will be stored at 37 ºC for 1 and 28 days and the HBSS will be changed weekly. The apparatus will be connected to a fluid conductive system and the fluid filtration rate will be measure over three 4-min periods at 1-min intervals. The data will be submitted to Parametric tests to verify if the samples were normally distributed. Thus Analysis of variance (ANOVA) with P = 0.05 and Tukey post hoc test will be used to perform multiple comparison tests. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VIAPIANA, R.; FLUMIGNAN, D. L.; GUERREIRO-TANOMARU, J. M.; CAMILLERI, J.; TANOMARU-FILHO, M. Physicochemical and mechanical properties of zirconium oxide and niobium oxide modified Portland cement-based experimental endodontic sealers. International Endodontic Journal, v. 47, n. 5, p. 437-448, MAY 2014. Web of Science Citations: 28.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.