Purification, immobilization and characterization of fungal lipase with potential for environmental applications

• Grant number: 12/14615-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2012
• End date: January 31, 2013
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Fernanda Dell Antonio Facchini
• Supervisor: José M. Guisan
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Universidad Autónoma de Madrid (UAM), Spain
• Associated to the scholarship: 11/07452-9 - Bioprospection, production, purification, immobilization and characterization of fungal lipase with potential environmental applications, BP.DR

Abstract

Lipases are enzymes that have received great attention in recent years, mainly due to its wide application in industry and environment. This PhD project is against the Thematic Project titled "Bio-prospection of filamentous fungi holoenzymes producers with bio refinery application potential." The culture medium optimization to grow and the selection of a good microorganism lipase producer able to realize transesterification reactions have been performed. However, the next step will be held on stage abroad (Spain) and aims at the purification of lipase from Fusarium verticillioides in a single chromatographic step and the immobilization of the same in different hydrophobic supports with different degrees of hydrophobicity such as butyl, phenyl, octyl and decaoctyl. The adsorption of these lipases on different supports and polyacrylamide gels containing the enzyme adsorbed will be analyzed by SDS-electrophoresis. Moreover, enzymes will also be tested for stability after stabilization. (AU)