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In vitro evaluation of adherence of Staphylococcus epidermidis and Staphylococcus aureus after disinfection of ocular prosthesis acrylic resins.

Grant number: 12/13909-4
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Start date: February 01, 2013
End date: July 31, 2013
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Marcelo Coelho Goiato
Grantee:Amália Moreno
Supervisor: Elizabeth Fozo
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Institution abroad: University of Tennessee (UT), United States  
Associated to the scholarship:11/05864-8 - Evaluation of Staphylococcus spp adhesion ocular prosthesis acrylic resin and chromatic change of artificial iris buttons fabricated with different techniques and colors after chemical disinfection, BP.DR

Abstract

The disinfection of ocular prosthesis aims to control microbial biofilm formation and to prevent the inflammation and infection of the conjunctival endogenous microbiota associated with Staphylococcus spp. Biofilm development on the ocular prosthesis may induce frequent ocular bacteremia and antibiotics resistance. The aim of this study will be to assess the efficacy of disinfectant solutions against Staphylococcus epidermidis and Staphylococcus aureus biofilm cultures developed on ocular prosthesis acrylic resins surfaces. Acrylic samples were fabricated (1.0 cm in diameter and 0.3 cm in thickness) and divided according to disinfection solution used: distilled water (control); treated with neutral soap; treated with Opti-FreeÒ; treated with (0.5%, 2% and 4%) chlorhexidine; and treated with EfferdentÒ. Biofilm cultures were formed on liner surface for three different points: initial (24h), intermediate (48h) and mature (72h), and then the specimens were randomly assigned to one of disinfectant treatments. After the treatments, the specimens were vortexed to disrupt the biofilm, and residual cells were counted (cell/mL).Data will be subjected to statistical analysis and evaluated according to the appropriate statistical test.

News published in Agência FAPESP Newsletter about the scholarship:
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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
MORENO, AMALIA; DOS SANTOS, DANIELA MICHELINE; LAMARTINE DE MORAES MELO NETO, CLOVIS; LUIZ DE MELO MORENO, ANDRE; DE MAGALHAES BERTOZ, ANDRE PINHEIRO; GOIATO, MARCELO COELHO. In vitroevaluation of the effect of different disinfectants on the biofilm ofStaphylococcus epidermidisandStaphylococcus aureusformed on acrylic ocular prostheses. PLoS One, v. 15, n. 10, . (12/13909-4)