| Grant number: | 12/17797-6 |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| Start date: | November 10, 2012 |
| End date: | March 09, 2013 |
| Field of knowledge: | Biological Sciences - Immunology - Cellular Immunology |
| Principal Investigator: | Constance Oliver |
| Grantee: | Valéria Cintra Barbosa Lorenzi |
| Supervisor: | Carolina Lunderius Andersson |
| Host Institution: | Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Institution abroad: | Karolinska Institutet, Sweden |
Abstract Mast cells are of hematopoietic origin, mainly localized at the interface between the host and the external environment that play a role in many physiological and pathological processes, which some of them are allergy, inflammation, and host response to pathogens. Mast cells are among the first cells of the immune system to interact with antigens, toxins, and pathogens, and this interaction occurs mainly, by a series of pattern recognition receptors (PRRs). The C-type lectins (CLRs) are among the PRRs, which play an important role in the defense against fungi, exemplified by the fact that Dectin-1 and Dectin-2 recognize beta-glucans and mannoses, respectively, from cell wall fungi. The skin commensal yeast Malassezia is known as one of the inducing agents for the development of atopic eczema (AE) and, mast cells have been considered as contributors for the establishment of AE, since the number and degree of activation of mast cells in the lesional AE skin are higher than in healthy skin. Regarding the importance of mast cells in the immune response against pathogens, especially fungi, the central aim of this study is to investigate the profile of mast cell response(s) triggered by the recognition of Malassezia sympodialis through C-type lectin receptors (CLRs). For this study, it will be used BMMCs (mouse bone marrow-derived mast cells) and PMCs (peritoneal mast cells). To study mast cell activation through CLRs it will be used different receptor ligands (zymozan, zymozan depleted, curdlan and, extract or nanovesicles from M. sympodialis). To evaluate mast cell activation, it will be performed mediators release assays. The intracellular pathways used for cell signaling will be addressed by Western blot and, mast cell receptor (CLRs) expression will be assessed by QT-PCR and flow cytometry. To fully understand the function of mast cells in these conditions more knowledge about how the cells are activated has to be obtained. (AU) | |
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