Analysis of the participation and localization of CD100 during infection of macrophages by Leishmania (Leishmania) amazonensis

• Grant number: 12/15048-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2012
• End date: January 31, 2016
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Beatriz Simonsen Stolf
• Grantee: Mariana Kolos Galuppo
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Leishmaniasis is a disease caused by protozoan trypanosomatids of the genus Leishmania, which infect preferentially macrophages. The disease can present itself in cutaneous, mucocutaneous or visceral forms, and 350 million people worldwide are at risk of infection. Several factors influence the shape and severity of the disease, among which the species of Leishmania and the host immune response are the most important. Studies based on animal models susceptible and resistant to Leishmania show how the immune system of hosts with different profiles affects the survival of the parasite. Considering the importance of macrophages in the course of Leishmania infection, the potential role of CD100 (that we showed to be expressed by macrophages from BALB/c and C57BL/6 mice) in the modulation of macrophage activation, and our data that sCD100 increases the infectivity of this cell by the parasite, we aim to clarify how CD100 participates in macrophage infection by Leishmania. For this, we will analyze the expression and localization of the protein in macrophages infected or not by Leishmania and the role of the soluble molecule and the one expressed by the macrophage in the infection.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GALUPPO, Mariana Kolos. Functional characterization of CD100 / SEMA4D in macrophage infection by Leishmania (Leishmania) amazonensis.. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.