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Exression of cytokines and gorwth factors in the bone repair of defects produced by burs and surgical ultrassound

Grant number: 12/16764-7
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): November 01, 2012
Effective date (End): July 31, 2013
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Elcio Marcantonio Junior
Grantee:Jônatas Caldeira Esteves
Supervisor abroad: Giuseppe Intini
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Local de pesquisa : Harvard University, Boston, United States  
Associated to the scholarship:10/07712-8 - Bone healing around dental implants placed in surgical alveolus prepared using drills, piezosurgery and Er,Cr:YSGG laser - radiographic, biomechanical, histomorphometric and immunohistochemical study in rats, BP.DR


Surgical ultrassound is an effective device to perform accurate and selective osteotomies with no injury of nearby structures. According to literature, low intensity ultrassound waves induces local citokynes and growth factor releasing, which, in turn, stimulate bone healing. However, it is not known, if surgical ultrasound produces the same effect. Thus, the aim of this study is to evaluate, by Real Time PCR, the release of cytokines and bone growth factors during early periods of the healing of defects created by conventional burs in comparison to surgical ultrasound. For this purpose, thirty rats will be divided into two groups with 15 animals: Group I - Bur and Group II, Surgical Ultrasound. The animals will be anesthetized and the right tibiae metaphysis will be surgically exposed for creation of a 2 millimeters in diameter bone defect by using rotary bur in Group I and Surgical Ultrassound in Group II. Five animals per group will be sacrificed immediately after the surgery, at 7 and 14 post operative days. The specimens will be reduced and processed for total RNA extraction and quantification of mRNA of Runx2, BMP-2, BMP-4, Alkaline Phosphatase (ALP) and TGF-²2 in each period. The data obtained will be submitted to statistical analysis with a significance level of 5%. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ESTEVES, JONATAS CALDEIRA; MARCANTONIO, JR., ELCIO; DE SOUZA FALONI, ANA PAULA; GODOY ROCHA, FERNANDA REGINA; MARCANTONIO, ROSEMARY ADRIANA; WILK, KATARZYNA; INTINI, GIUSEPPE. Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysis. JOURNAL OF TRANSLATIONAL MEDICINE, v. 11, SEP 23 2013. Web of Science Citations: 25.

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