Effects of early treatment with low level laser therapy on the muscle skeletal repair

• Grant number: 12/11461-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: December 31, 2013
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Raquel Agnelli Mesquita Ferrari
• Grantee: Beatriz Guimarães Ribeiro
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Memorial. São Paulo , SP, Brazil

Abstract

The low level laser therapy (LLLT) is widely used in order to promote faster and better repair muscle with reference to different types of injury, however, studies on the prior application of the laser are scarce. The aim of this study is to evaluate the effect of LLLT prior to injury on the repair process and in particular on the expression of myogenic regulatory factors MyoD and myogenin and the matrix metalloproteinase MMP-2. For that, will be used 100 Wistar rats divided into 05 groups: (G1) Control, (G2) Sham; (G3) LLLT immediately prior to muscle injury TAD; (G4) No lesion with LLLT immediately prior to sacrifice; (G5) Only injury without treatment with LLLT. The crioinjury applications consist of two rod cooled in liquid nitrogen directly into the TA muscle. Groups 3-5 will be assessed at 1, 3 and 7 days after injury. The LLLT will be performed using the GaAlAs laser (» 780nm) with the density energy of 5 J/cm², output power of 20 mW and time of 10 seconds. For the analysis of expression of myogenic factors will be extracted total RNA from TA muscle and will get the cDNA for the realization of real-time PCR using primers specific for each factor cited and MMP2. To assess the activity of MMP2 is used technique for detecting zymography gelatinase activity in the different groups. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, BEATRIZ GUIMARAES; ALVES, AGNELO NEVES; DIAS DOS SANTOS, LUCAS ANDREO; SANTOS FERNANDES, KRISTIANNE PORTA; CANTERO, TATIANE MATARAZZO; GOMES, MARIANA TEIXEIRA; FRANCA, CRISTIANE MIRANDA; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. The effect of low-level laser therapy (LLLT) applied prior to muscle injury. Lasers in Surgery and Medicine, v. 47, n. 7, p. 571-578, SEP 2015. (14/12381-1, 12/11461-6, 12/25198-5, 13/21540-3)

ALVES, AGNELO NEVES; RIBEIRO, BEATRIZ GUIMARES; SANTOS FERNANDES, KRISTIANNE PORTA; COSTA SOUZA, NADHIA HELENA; ROCHA, LILIA ALVES; NUNES, FABIO DAUMAS; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Comparative effects of low-level laser therapy pre- and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair. Lasers in Medical Science, v. 31, n. 4, p. 679-685, MAY 2016. (14/12381-1, 13/21540-3, 12/11461-6, 11/04452-8, 11/17638-2)