Analysis of the variability and transmission of begomovirus by specimens belonging to Bemisia tabaci New World 2 group

• Grant number: 12/17373-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: July 31, 2014
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Renate Krause Sakate
• Grantee: Bruno Rossitto de Marchi
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The whiteflies (Bemisia tabaci) are recognized as one of the most important pest of the century, mainly because they are vectors of begomoviruses. They are insects with high biological variability and are classified as a complex of species sub-divided into 11 sub groups containing at least 29 species morphologically indistinguishable. Between these species, only Middle East-Asia Minor 1 (MEAM1), New World 1 (NW1) and New World 2 (NW2) were detected in Brazil so far. The species MEAM1, also known as biotype B, is recognized as the largest of worldwide distribution, while species native to the Americas belonging to the group New World are still poorly studied, mainly because they have been discovered (as in NW2) recently and its genetic variability and epidemiological importance in the transmission of begomovirus is still unknown. In this work, we intend to evaluate the capacity of New World 2 species to transmit begomovirus and the number of haplotypes found in the sampled areas. Whiteflies at different locations in the State of São Paulo will be collected and the mitochondrial gene cytochroma oxidase I (mtCOI) analyzed by RFLP and sequencing for identification of species and variability analysis. The presence of begomovirus directly into the insect will be evaluated by RCA-PCR and will indicate whether the species NW2 is a vector or not of begomovirus. A pure colony of the species NW2 will be established to enable assays of begomovirus transmission by the insect. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, LEONARDO DA F.; MARUBAYASHI, JULIO M.; DE MARCHI, BRUNO R.; YUKI, VALDIR A.; PAVAN, MARCELO A.; MORIONES, ENRIQUE; NAVAS-CASTILLO, JESUS; KRAUSE-SAKATE, RENATE. Indigenous American species of the Bemisia tabaci complex are still widespread in the Americas. Pest Management Science, v. 70, n. 10, SI, p. 1440-1445, OCT 2014. (13/12377-1, 12/17373-1, 10/17313-3)

BARBOSA, LEONARDO DA FONSECA; YUKI, VALDIR ATSUSHI; MARUBAYASHI, JULIO MASSAHARU; DE MARCHI, BRUNO ROSSITTO; PERINI, FERNANDO LUIS; PAVAN, MARCELO AGENOR; DE BARROS, DANIELLE RIBEIRO; GHANIM, MURAD; MORIONES, ENRIQUE; NAVAS-CASTILLO, JESUS; et al. First report of Bemisia tabaci Mediterranean (Q biotype) species in Brazil. Pest Management Science, v. 71, n. 4, p. 501-504, APR 2015. (13/12377-1, 12/17373-1)

BARBOSA, LEONARDO DA F.; MARUBAYASHI, JULIO M.; DE MARCHI, BRUNO R.; YUKI, VALDIR A.; PAVAN, MARCELO A.; MORIONES, ENRIQUE; NAVAS-CASTILLO, JESUS; KRAUSE-SAKATE, RENATE. Indigenous American species of the Bemisia tabaci complex are still widespread in the Americas. Pest Management Science, v. 70, n. 10, p. 6-pg., 2014-10-01. (10/17313-3, 12/17373-1, 13/12377-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARCHI, Bruno Rossitto de. Associação de begomovírus e crinivírus com bemisia tabaci espécie new world 2 e Trialeurodes vaporariorum. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu Botucatu.