Bemisia tabaci in Brazil: competitive displacement between criptic species, susceptibility to insecticides, next generation sequencing analysis, virus influence on insect biology and the presence of the endosymbiont Hamiltonella in virus transmission

• Grant number: 17/21588-7
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2018 - February 29, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Renate Krause Sakate
• Grantee: Renate Krause Sakate
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The whitefly (Bemisia tabaci) is one of the most important pests in the world, especially being a vector of plant viruses. B. tabaci is classified in a complex of cryptic species. In Brazil, the invasive species Middle East-Asia Minor 1, MEAM1 (known as biotype B) and Mediterranean, MED (known as biotype Q) are the main responsible for great yield losses. MEAM1 was first reported in 1992 while MED was reported more recently; in 2013 in Rio Grande do Sul State and 2014 in São Paulo and Paraná States. Unfortunately, this pest is wide spreading in our country by ornamental plants and invading areas that MEAM1 is predominant. To date, the behaviour of MED is not known and this project has as goals: evaluate the competitive displacement between MED and MEAM1 in different hosts; evaluate biological behaviour, such as life cycle, oviposition, number of eggs/nymphs, survival and adults viability in virus-infected plants and non-virus-infected plants; analyse insecticides susceptibility (on egg, nymphs and adults stages) and the diversity of several B. tabaci population from Brazil by Next Generation Sequencing analysis. Furthermore, areas that we have reported MED in São Paulo and Paraná States will be monitored to verify the adaptation of this pest on field crops. The data obtained will address if MED is able to displace MEAM1; which species has more ability to displace another whereas there are several crops in the field and which insecticides are better to control each species. Moreover, Next Generation Sequencing analysis of B. tabaci populations from Brazil will provide data about genetic diversity, endosimbionts presence and virus associated to each cryptic species. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, FELIPE BARRETO; MULLER, CRISTIANE; BELLO, VINICIUS HENRIQUE; MARANHO WATANABE, LUIS FERNANDO; DE MARCHI, BRUNO ROSSITTO; FUSCO, LUCAS MACHADO; RIBEIRO-JUNIOR, MARCOS ROBERTO; MINOZZI, GUILHERME BARBOSA; VIVAN, LUCIA MADALENA; TAMAI, MARCO ANTONIO; et al. Effects of cowpea mild mottle virus on soybean cultivars in Brazil. PeerJ, v. 8, AUG 31 2020. (17/21588-7)

BELLO, VINICIUS HENRIQUE; WATANABE, LUIS FERNANDO MARANHO; FUSCO, LUCAS MACHADO; DE MARCHI, BRUNO ROSSITTO; DA SILVA, FELIPE BARRETO; GORAYEB, EDUARDO SILVA; MOURA, MONIKA FECURY; DE SOUZA, ISABELA MORCILO; MULLER, CRISTIANE; SALAS, FERNANDO JAVIER SANHUEZA; et al. Outbreaks ofBemisia tabaciMediterranean species in vegetable crops in Sao Paulo and Parana States, Brazil. BULLETIN OF ENTOMOLOGICAL RESEARCH, v. 110, n. 4, p. 487-496, AUG 2020. (17/21588-7, 18/18274-3)

MARANHO WATANABE, LUIS FERNANDO; BELLO, VINICIUS HENRIQUE; DE MARCHI, BRUNO ROSSITTO; DA SILVA, FELIPE BARRETO; FUSCO, LUCAS MACHADO; SARTORI, MARIA MARCIAPEREIRA; PAVAN, MARCELO AGENOR; KRAUSE-SAKATE, RENATE. Performance and competitive displacement of Bemisia tabaci MEAM1 and MED cryptic species on different host plants. CROP PROTECTION, v. 124, OCT 2019. (17/21588-7)

BELLO, VINICIUS HENRIQUE; MARANHO WATANABE, LUIS FERNANDO; SANTOS, BEATRIZ ROSA; MARUBAYASHI, JULIO MASSAHARU; YUKI, VALDIR ATSUSHI; DE MARCHI, BRUNO ROSSITTO; PAVAN, MARCELO AGENOR; KRAUSE-SAKATE, RENATE. Evidence for increased efficiency of virus transmission by populations of Mediterranean species of Bemisia tabaci with high Hamiltonella prevalence. PHYTOPARASITICA, v. 47, n. 2, p. 293-300, APR 2019. (17/21588-7)

XAVIER, CESAR A. D.; NOGUEIRA, ANGELICA MARIA; BELLO, VINICIUS HENRIQUE; MARANHO WATANABE, LUIS FERNANDO; CARNEIRO BARBOSA, TARSIANE MARA; ALVES JUNIOR, MIGUEL; BARBOSA, LEONARDO; BESERRA-JUNIOR, JOSE E. A.; BOARI, ALESSANDRA; CALEGARIO, RENATA; et al. Assessing the diversity of whiteflies infesting cassava in Brazil. PeerJ, v. 9, JUL 15 2021. (17/21588-7)

GORAYEB, EDUARDO SILVA; WATANABE, LUIS FERNANDO MARANHO; PEREIRA, YAGO ALEXANDRE BARBI; DOVIGO, LEONARDO HIPOLITO; BELLO, VINICIUS HENRIQUE; SOUZA, ISABELA MORCILO DE; CRUCIOL, GIOVANA CAROLINA DOURADO; VICENTIN, EDUARDO; SARTORI, MARIA MARCIA PEREIRA; KRAUSE-SAKATE, RENATE. Performance and preference of Bemisia tabaci on cucumbers: Understanding the recent outbreaks of Mediterranean cryptic species in Brazil. CROP PROTECTION, v. 143, MAY 2021. (17/50222-0, 19/08377-2, 17/21588-7, 18/18274-3)

MULLER, CRISTIANE; MARUBAYASHI, JULIO MASSAHARU. Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil. SCIENTIFIC REPORTS, v. 8, OCT 1 2018. (17/21588-7)

DE MARCHI, BRUNO ROSSITTO; KINENE, TONNY; WAINAINA, JAMES MBORA; KRAUSE-SAKATE, RENATE; BOYKIN, LAURA. Comparative transcriptome analysis reveals genetic diversity in the endosymbiont Hamiltonella between native and exotic populations of Bemisia tabaci from Brazil. PLoS One, v. 13, n. 7, JUL 27 2018. (17/21588-7)

MARANHO WATANABE, LUIS FERNANDO; BELLO, VINICIUS HENRIQUE; DE MARCHI, BRUNO ROSSITTO; PEREIRA SARTORI, MARIA MARCIA; PAVAN, MARCELO AGENOR; KRAUSE-SAKATE, RENATE. Performance of Bemisia tabaci MEAM1 and Trialeurodes vaporariorum on Tomato chlorosis virus (ToCV) infected plants. Journal of Applied Entomology, v. 142, n. 10, p. 1008-1015, DEC 2018. (17/21588-7)

KRAUSE-SAKATE, RENATE; MARANHO WATANABE, LUIS FERNANDO; GORAYEB, EDUARDO SILVA; DA SILVA, FELIPE BARRETO; ALVAREZ, DANIEL DE LIMA; BELLO, VINICIUS HENRIQUE; NOGUEIRA, ANGELICA MARIA; DE MARCHI, BRUNO ROSSITTO; VICENTIN, EDUARDO; RIBEIRO-JUNIOR, MARCOS ROBERTO; et al. Population Dynamics of Whiteflies and Associated Viruses in South America: Research Progress and Perspectives. INSECTS, v. 11, n. 12, DEC 2020. (17/21588-7, 18/18274-3)