Evaluation of capillary electrophoresis to quantificate nebivolol on solid pharmaceutical formulation and on enantioselective binding to human plasma proteins.

• Grant number: 12/16597-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: July 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Cristiane Masetto de Gaitani
• Grantee: Ana Débora Nunes Pinheiro
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Nebivolol is an antihypertensive specific ²1-adrenergic block drug. It is commercialized as a racemic mixture, D(+)(-)-nebivolol e L(-)(-)-nebivolol. The both enantiomers have different pharmacological activities, which suggests a different pharmacokinetics as well. Nebivolol metabolism is complex and it is subjected to genetic polymorphism of CYP2D6, responsible enzime by nebivolol metabolism. The kinect of nebivolol is enantiosselective on pacients extensive metabolisers, but not on poor metabolisers. Despite of this information, a few is known about nebivolol enantiosselective binding to plasma proteins. It is known that nebivolol binding is around 98% though. A technique used to quantify drugs on the drug-protein binding is capillary electrophoresis (CE). This technique allows the analysis of many compounds and it has been used to chiral drugs analysis. Therefore, the aim of this work is to evaluate enantioselective binding of nebivolol to plasma proteins by CE. The lack of information about this subject, suggests that this is an unpublished work.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PINHEIRO, Ana Débora Nunes. Evaluation of capillary electrophoresis for the nebivolol quantification in solid pharmaceutical forms and enantioselective analysis of nebivolol binding to plasmatic proteins. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.