Effect of Melaleuca alternifolia essential oil and its main component Terpinen-4-ol against clinical isolates of Candida albicans resistant

• Grant number: 12/15680-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2013
• End date: March 31, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Renata Serignoli Francisconi
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The essential oil of Melaleuca alternifolia (TTO) is an herbal antifungal action. This study will evaluate the efficacy of TTO and its main component Terpinen-4-ol against resistant clinical isolates and reference strains of Candida albicans and its effect on biofilm developed in acrylic resin blocks. This study will realized in three stages: 1. Identification of the MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration) of TTO and Terpinen-4-ol in the planktonic form of C. albicans at the concentrations to be determined before the experiment. Analysis of different concentrations of oil on biofilm SSD (monoespécie) of C. albicans developed in the microtiter plate. Nystatin will be used as control. The microorganisms will be quantified in CFU / ml and assessed for metabolic activity of cells by the XTT method. 3. Biofilm formation developed in specimens of acrylic resin that will be submitted to the following solutions: TTO and Terpinen-4-ol (only in inhibitory concentration determined in the previous phase), PBS (positive control) and nystatin (Sigma). After this test, analyzes will be performed by XTT test and CLSM (confocal microscopy) to determine viable and non viable cells in the biofilm.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RENATA SERIGNOLI FRANCISCONI; PATRICIA MILAGROS MAQUERA HUACHO; CAROLINE CORADI TONON; ESTER ALVES FERREIRA BORDINI; MARÍLIA FERREIRA CORREIA; JANAÍNA DE CÁSSIA ORLANDI SARDI; DENISE MADALENA PALOMARI SPOLIDORIO. Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans. Brazilian Oral Research, v. 34, 2020-06-05. (12/15680-4)

FRANCISCONI, RENATA SERIGNOLI; MAQUERA HUACHO, PATRICIA MILAGROS; TONON, CAROLINE CORADI; FERREIRA BORDINI, ESTER ALVES; CORREIA, MARILIA FERREIRA; ORLANDI SARDI, JANAINA DE CASSIA; PALOMARI SPOLIDORIO, DENISE MADALENA. Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans. BRAZILIAN ORAL RESEARCH, v. 34, p. 9-pg., 2020-01-01. (12/15680-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FRANCISCONI, Renata Serignoli. Efeito do óleo essencial de Melaleuca alternifolia e de seu principal componente Terpinen-4-ol sobre isolados clínicos de Candida albicans resistentes. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.