Enzymatic characterization and pharmacological activity of sPLA2 isolated from whole venom and human thrombin in the presence of extract of Laguncularia racemosa and its partitions

• Grant number: 12/21764-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2013
• End date: December 31, 2014
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Marcos Hikari Toyama
• Grantee: Caroline Fabri Bittencourt Rodrigues
• Host Institution: Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

The therapeutic potential of many plant species is assigned to biologically active compounds such as flavonoids, alkaloids, terpenoids, which have been reported as promising for the development of new anti-inflammatory drugs and other therapeutic applications. Typical mangrove plant species exhibit different chemical compositions in particular metabolites such as polyphenols, terpenoids, polysaccharides and other compounds at lower concentrations, have been demonstrated some important pharmacological actions such as anti-inflammatory activity, antioxidant, antitumor, antifungal and antibacterial. These studies showed the presence of important bioactive compounds in plants of the swamp, although they are still few and do not have knowledge about the real potential of plant species as sources of bioactive. The goal of this project is to verify the action of methanol extract and ethanolic crude and their respective partitions (dichloromethane, ethyl acetate) sheet Laguncularia racemosa species on the enzymatic activity of thrombin and human secretory phospholipase A2 isolated from Crotalus terrificus durissis. In addition to check the pharmacological potential, the type of inhibition and minimum inhibitory concentration, it is intended to check the possible structural alterations induced by these compounds on the native pure protein by monitoring the tryptophan fluorescence spectrum, circular dichroism scan from 260 to 320nm in order to verify possible loss of tertiary structure. Besides the biochemical characterization, the objective is to evaluate the anti-inflammatory effect on sPLA2 rattlesnake, in the absence and presence of different extracts and their partitions.(AU)