Entire sequencing of high-molecular weight resistance plasmids carrying the most frequent antimicrobial resistance genes found in Enterobacteriaceae

• Grant number: 12/24864-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 13, 2013
• End date: December 12, 2013
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Ana Lúcia da Costa Darini
• Grantee: Renata Galetti
• Supervisor: Alessandra Carattoli
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Istituto Superiore di Sanità (ISS), Italy

Abstract

Bacterial resistance to antibiotics is mainly due to acquisition of extrachromosomal elements of other bacteria, including transposons, integrons, plasmids and prophages, wich are exchanged between bacteria by processes such as transformation, conjugation, and transduction. Plasmids are the most relevant mobile genetic elements due to their ability to promote horizontal gene transfer between different bacterial genera by conjugation. The main ²-lactams and fluoroquinolones resistance genes are located on plasmids as other resistance determinants. Some methods are used for plasmids typing in Enterobacteriaceae and Acinetobacter spp.- PCR-based replicon typing (PBRT and AB-PBRT, respectively) was developed and are accomplished, but some plasmids are difficult to identify and classify, as well as they can not be typed by these schemes, and more "aggressive" approaches as the complete sequencing of DNA are necessary. In this study pyrosequencing technique will be applied that allows complete sequencing of the plasmids. The strategy used in Italy to Enterobacteriaceae plasmids could be used in further studies of plasmids sequencing of non-fermenting bacterial, as Pseudomonas aeruginosa. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARATTOLI, A.; FORTINI, D.; GALETTI, R.; GARCIA-FERNANDEZ, A.; NARDI, G.; ORAZI, D.; CAPONE, A.; MAJOLINO, I.; PROIA, A.; MARIANI, B.; et al. Isolation of NDM-1-producing Pseudomonas aeruginosa sequence type ST235 from a stem cell transplant patient in Italy, May 2013. EUROSURVEILLANCE, v. 18, n. 46, p. 5-7, NOV 14 2013. (12/24864-1)