Septin of Chlamydomonas reinhardtii: studies focusing its expression and function

• Grant number: 12/21259-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2013
• End date: November 06, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Ana Paula Ulian de Araujo
• Grantee: Heloisa Ciol
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated scholarship(s): 14/02618-4 - Septin of Chlamydomonas reinhardtii: studies focused on its function and expression, BE.EP.DD

Abstract

Septins are part of a protein family of guanine nucleotide binding that have been found in several eukaryotes, but never in plants. These proteins were reported playing roles in cytokinesis, cell structure, and exocytosis, but little is known about its mode of action. Moreover, septins show the ability to interact with other septins to polymerize in hetero filaments assembling higher order structures. This project aims the functional characterization of the septin of the unicellular Chlamydomonas reinhardtii algae, a model eukaryote that diverged early from a common ancestor of the plants and metazoans. As result we expect to help understand the fundamental role this protein plays in C. reinhardtii and also clarify the controversial issue about the existence of functional homo filaments of septins. Chlamydomonas is an attractive model for the study of septins in vivo, because in addition to the well established protocols for cultivation and transformation, it presents a single septin, unlike other eukaryotes studied so far. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, ANDRESSA P. A.; PEREIRA, HUMBERTO M.; ZERAIK, ANA E.; CIOL, HELOISA; FERREIRA, FREDERICO M.; BRANDAO-NETO, JOSE; DEMARCO, RICARDO; NAVARRO, MARCOS V. A. S.; RISI, CRISTINA; GALKIN, VITOLD E.; et al. Filaments and fingers: Novel structural aspects of the single septin from Chlamydomonas reinhardtii. Journal of Biological Chemistry, v. 292, n. 26, p. 10899-10911, JUN 30 2017. (11/10152-7, 14/15546-1, 12/21259-0, 12/14223-9, 13/20715-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CIOL, Heloisa. Septin of Chlamydomonas reinhardtii: studies focused on its expression and function. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.