Functional, biochemical and molecular study of Purinergic P1 and P2 receptors in atria of Normotensive and Hypertensive Rats

• Grant number: 12/22763-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Aron Jurkiewicz
• Grantee: Juliano Quintella Dantas Rodrigues
• Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Purine, such as ATP and pyrimidine, and UTP are stored with norepinephrine synaptic vesicles and released from sympathetic nerve terminals. The action of these nucleotides in these synapses is mediated by P1 and P2 purinergic receptors expressed in various tissues. Studies show the role of the purinergic system in cardiovascular regulation. Both nucleotides stimulate vasoconstriction and vasodilation, muscle cell growth and vascular endothelial cells, angiogenesis and vascular remodeling. Accordingly as the cellular and molecular mechanisms involving purinoceptores and heart are not concluded in hypertension has become important to investigate the role of these receptors in the regulation of atrial function in normotensive and hypertensive animals. We isolated the right atria (RA) and left (AE) of normotensive Wistar rats (NWR) and spontaneously hypertensive rats (SHR) from 4 to 6 months, conducting a study functional, biochemical and molecular investigating whether the signaling pathway and the P1 and P2 purinergic receptors exhibit any change in hypertension. Thus, we use agonists and antagonists selective for characterizing the role of purinergic receptors in the regulation of heart function. We will study functional transient calcium ions involved in the positive inotropic effect produced by ATP and UTP in AE and AD isolates NWR and SHR. Through the technique of real-time PCR we will measure the difference in receptor expression in SHR. The technique of Western blotting and immunohistochemistry we will try to investigate the production and location of these receptors in the atrial tissue of both species.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, JULIANO Q. D.; CAMARA, HENRIQUE; JURKIEWICZ, ARON; GODINHO, ROSELY O.. Increased Gi protein signaling potentiates the negative chronotropic effect of adenosine in the SHR right atrium. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, v. 391, n. 5, p. 513-522, MAY 2018. (13/20402-6, 12/22763-3, 15/07019-4, 12/24828-5)

DANTAS RODRIGUES, JULIANO QUINTELLA; CAMARA, HENRIQUE; DA SILVA JUNIOR, EDILSON DANTAS; GODINHO, ROSELY OLIVEIRA; JURKIEWICZ, ARON. Intrinsic Adaptation of SHR Right Atrium Reduces Heart Rate. Journal of Cardiovascular Pharmacology, v. 74, n. 6, p. 542-548, DEC 2019. (12/22763-3, 15/07019-4, 12/24828-5)