Deletion of putative invasin and DnaK genes from avian pathogenic Escherichia coli (APEC) strain SCI07 to study their role in pathogenicity and obtain attenuated vaccine strains

• Grant number: 12/23289-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Wanderley Dias da Silveira
• Grantee: Renu Verma
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Brazil is the third larger producer and the larger exporter of poultry meat world-wide. The production is estimated to be near about 12 millions of ton. Approximately 4 millions of tons is exported yearly, by which earning income is about US$ 6 billion. Species of avian pathogenic Escherichia coli (APEC) are agents of significant degrees of morbidity and mortality for poultry and laying hens, causing losses to the poultry industry. A plethora of years, Prof. Wanderley and his colleagues have published works on APEC species for characterizing them with respect to their biological characteristics related to pathogenicity (adhesion and invasion of cell species ability, resistance to antimicrobials, expression of fimbriae and flagella), genetics (existence of pathogenicity related genes in plasmids and chromosomes) and many more characteristics. In this proposed work, we aim to study on two important genes, which are known as putative invasin and DnaK, potentially involved in the pathogenicity of an APEC. By deletion of these two genes, we would be able to reduce pathogenicity process in APEC swollen head syndrome strain SCI07 and construct attenuated strains to be used as vaccines against APEC infections as well. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VERMA, RENU; GALVAO ROJAS, THAIS CABRERA; MALUTA, RENATO PARIZ; LEITE, JANAINA LUISA; NAKAZATO, GERSON; DE SILVEIRA, WANDERLEY DIAS. Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro. MICROBIOLOGICAL RESEARCH, v. 214, p. 28-36, 2018. (12/23289-3, 12/09655-7, 12/05073-3, 15/03860-6, 13/09167-5, 14/04860-7)

VERMA, RENU; GALVAO ROJAS, THAS CABRERA; MALUTA, RENATO PARIZ; LEITE, JANANA LUISA; MENDES DA SILVA, LIVIA PILATTI; NAKAZATO, GERSON; DA SILVEIRA, WANDERLEY DIAS. Fimbria-Encoding Gene yadC Has a Pleiotropic Effect on Several Biological Characteristics and Plays a Role in Avian Pathogenic Escherichia coli Pathogenicity. Infection and Immunity, v. 84, n. 1, p. 187-193, JAN 2016. (12/23289-3, 12/04391-1, 12/05073-3, 13/09167-5, 14/04860-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VERMA, Renu. Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.