In this project the investigation of different metal complexes (copper and zinc) with diverse imine ligands as probable proteasome inhibitors will be carried out. Proteasome is a proteolytic protein, capable of degradating different cellular proteins. In these studies EPR and CD spectroscopies will be used to verify possible interactions between some prepared complexes and the proteasome. The inhibitory activity of such complexes will be monitored by usual methods already described in the literature, utilizing specific substrates, depending on the focused activity (chymotrypsine, trypsine or caspase-like activities). Each one of these activities seems to be associated to different catalytic site in the protein, and will help in the elucidation of the mechanism of action of the studied metal complexes.
News published in Agência FAPESP Newsletter about the scholarship: