Chemical characterization and comparision of secondary metabolic profile of Conchocarpus fontanesianus (Rutaceae) in two regions of Atlantic Rainforest in São Paulo State

• Grant number: 13/05480-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2013
• End date: May 31, 2014
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Maria Claudia Marx Young
• Grantee: Rodrigo Sant'Ana Cabral
• Supervisor: Jean-Luc Wolfender
• Host Institution: Instituto de Botânica. Secretaria de Meio Ambiente, Infraestrutura e Logística (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: Université de Genève, Switzerland
• Associated to the scholarship: 11/12515-0 - Chemical diversity and biological potential of Conchocarpus fontanesianus (A. St.-Hil.) Kallunki & Pirani (Rutaceae), BP.DR

Abstract

The plant kingdom is responsible for a large portion of the chemical diversity recorded in literature and, since the beginnings of civilization, has contributed significantly to the supply of useful substances to humans. It is still an untapped reservoir of new molecules with potential therapeutic interest and only a relative small percentage of the 400,000 known plant species have been studied on a phytochemical or a pharmacological viewpoint. The chemical screening or metabolite profiling is aimed at distinguishing between already known compounds (dereplication) and new molecules directly in crude plant extracts. The potential of chemical screening strategy has been considerably increased by the recent development of hyphenated techniques, which are able to provide efficient separation of the metabolites and valuable structural information at the same time. By coupling HPLC with both mass spectrometry (LC/MS) and UV diode array detection (LC/UV), a large amount of data can be obtained on the constituints of a plant extract before starting any isolation work. Moreover, the recent introduction of the coupling of HPLC with nuclear magnetic resonance (LC/NMR) allows the complete structure of numerous plant metabolites to be elucidated on-line. The Conchocarpus genus, belonging to the Rutaceae family, consists of shrubs or small trees, with 45 species that are distributed from Nicaragua to northern of Bolivia and Brazil. C. fontanesianus, has small tree structure, reaching 1-3 meters tall, is native, endemic and distributed in the states of Rio de Janeiro and São Paulo, found in regions of Atlantic Rainforest. This project aims to analyze the profile of secondary metabolic of C. fontanesianus through analysis by hyphenated techniques of extracts and fractions of stems and leaves of individuals collected in two regions of the Atlantic Rainforest in São Paulo State. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CABRAL, RODRIGO SANT'ANA; ALLARD, PIERRE-MARIE; MARCOURT, LAURENCE; MARX YOUNG, MARIA CLAUDIA; QUEIROZ, EMERSON FERREIRA; WOLFENDER, JEAN-LUC. Targeted Isolation of Indolopyridoquinazoline Alkaloids from Conchocarpus fontanesianus Based on Molecular Networks. Journal of Natural Products, v. 79, n. 9, p. 2270-2278, SEP 2016. (13/05480-0)