Use of elastin matrix derived from the auricular cartilage defect repair in the rat cranial

• Grant number: 12/24663-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2013
• End date: April 30, 2014
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Marcelo Rodrigues da Cunha
• Grantee: Ewerton Alexandre Galdeano
• Host Institution: Centro Universitário Padre Anchieta (UniAnchieta). Sociedade Padre Anchieta de Ensino (SPAE). Jundiaí , SP, Brazil

Abstract

Through the difficulties and limitations encountered in bone reconstructive surgery, tissue engineering has been highlighted in regenerative therapies through the manufacturing and development of new bio-devices to stimulate proper bone healing in clinical situations where there is a need for grafting. Thus, new biomaterials are constantly being tested to serve as support for the three-dimensional growth of new tissue in the quality of bone injury. The objective of this study is to evaluate the osteogenic potential of a new elastin matrix derived from bovine cartilage. For this, 24 mice will be used where the surgical procedure will be subjected to create a defect with 5mm diameter in the left parietal bone of the skull that can be implanted matrices elastin. After 3 and 8 weeks after surgery, the animals will be euthanized and samples of the receiving area of the implant matrix will be subjected to routine histological methods. The macroscopic analysis will be performed, radiological, histological, and morphometric statistics to study the biocompatibility of the matrix as well as its ability to promote bone formation essential for the repair of the defect.(AU)