Periodontal tissues alterations in two models of periodontal disease induction in mice: histological and computed microtomography analysis

• Grant number: 12/11860-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2013
• End date: April 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Joni Augusto Cirelli
• Grantee: Gustavo Boze Toffoli
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Periodontitis is an infectious disease characterized by the destruction of alveolar bone tissue, mediated and modulated by the host response against the microorganisms from the biofilm. Understanding the origin and evolution of the alveolar bone resorption is one of the primary goals of periodontal research, especially to stop the disease progression and improve future treatments. For this, the development of experimental models is important and a necessary step before entering into clinical trials with new treatment therapies. Therefore, the aim of this study will be to characterize and evaluate tissue changes caused by two experimental models of periodontal disease in mice. For this, will be used C57/BL6 wild-type mice (WT), females, distributed into 4 experimental groups and sacrificed at 30 and 45 days after periodontal disease induction (n=6 animals/period/group). Group C will be the negative control group; Group L periodontal disease will be induced by a ligature around first maxillary molars; in Groups, G-Pg and G-PgFn oral gavage with Porphyromonas gingivalis and Fusobacterium nucleatum + Porphyromonas gingivalis will be performed, respectively. The samples will be used for the analysis of the immune-inflammatory process in the gingival tissue by histological descriptive analysis and stereometric analyses. The alveolar bone loss will be evaluated by micro-computerized tomography (¼CT). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MOLON, RAFAEL SCAF; MASCARENHAS, VINICIUS IBIAPINA; DE AVILA, ERICA DORIGATTI; FINOTI, LIVIA SERTORI; TOFFOLI, GUSTAVO BOZE; PALOMARI SPOLIDORIO, DENISE MADALENA; SCAREL-CAMINAGA, RAQUEL MANTUANELI; TETRADIS, SOTIRIOS; CIRELLI, JONI AUGUSTO. Long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 6, p. 1203-1216, JUL 2016. (12/11860-8)