Comparative study of DNA extraction in different biological material sources to performance the polymerase chain reaction (PCR) in the diagnosis of Polycystic Kidney Disease in Persian cats

• Grant number: 13/06471-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2013
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Bruno Cogliati
• Grantee: Natalia Cavalca Cardoso
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The autosomal dominant polycystic kidney disease (ADPKD) is one of the congenital hereditary diseases that most affects cats, mainly Persian breed, which is characterized by development of cysts in renal parenchyma and, eventually, in other organs, leading to a commitment of their functions and a guarded to poor prognostic. The ADPKD in felines has, as main genetic change, transversion of cytosine for adenine nucleotide at position 3284 of exon 29 of PKD1 (Polycystic Kidney Disease 1) gene, resulting in a premature mRNA stop codon. First of all, it is known that these cysts may occur at any age and silently, hampering the early diagnostic, once the ultrasound is one of the most classic exams to identify it. However, most often the image exam is performed only when the animal is already showing any clinical sign at advanced stages of the disease, such as polydipsia, hematuria, polyuria. Therefore, the genetic evaluation is still considerate the safest and the most efficient test for the early diagnostic of APKD in cats. On the other hand, the more practical is to collect the biological material for genetic evaluation, the greater is the chance of supporting the diagnostic exam by the owners and the stress submitted by cats will be lower. Nowadays, the golden standard for genomic DNA extraction is by means of blood, invasive method that requires active participation of the veterinarian and, often, relies on animal collaboration. Thus, this work has as main goal to test and to standardize the genomic DNA extraction from biological materials for easy collection in Persian cats, such as oral epithelial mucosa cells and hair, using different extraction methods. To check each sample efficiency, the quantity and quality of extracted genetic material will be evaluated in comparison with blood from the same animals, in addition to performing PCR and RFLP for identification of exon 29 mutation in PKD1 feline gene. These data may help the owners and veterinarians in the early diagnostic of APKD, besides assisting to control and to eradicate the disease in Persian cats.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JULIANA MARIOTTI GUERRA; ARINE PELLEGRINO; ALEXANDRE GONÇALVES TEIXEIRA DANIEL; MARIANA FERREIRA DE FREITAS; NATÁLIA CAVALCA CARDOSO; REBECCA BASTOS PESSOA; GABRIEL GARONE DE LUCCA; MARIA HELENA MATIKO AKAO LARSSON; LUIZ FERNANDO ONUCHIC; FELISBINA LUISA QUEIROGA; et al. Achados estruturais e funcionais cardíacos em gatos persas com doença renal policística autossômica dominante. Ciência Rural, v. 50, n. 9, 2020-08-07. (12/04990-2, 12/19614-6, 13/06471-5)

J. M. GUERRA; N. C. CARDOSO; A. G. T. DANIEL; L. F. ONUCHIC; B. COGLIATI. Prevalência da doença renal policística autossômica dominante em gatos Persas e raças relacionadas no Brasil. Brazilian Journal of Biology, v. 81, n. 2, p. 392-397, 2020-05-29. (13/06471-5, 12/19614-6)

GUERRA, JULIANA M.; FREITAS, MARIANA F.; DANIEL, ALEXANDRE G. T.; PELLEGRINO, ARINE; CARDOSO, NATALIA C.; DE CASTRO, ISAC; ONUCHIC, LUIZ F.; COGLIATI, BRUNO. Age-based ultrasonographic criteria for diagnosis of autosomal dominant polycystic kidney disease in Persian cats. JOURNAL OF FELINE MEDICINE AND SURGERY, v. 21, n. 2, p. 156-164, FEB 2019. (13/06471-5, 12/19614-6)