Morpho-function analysis of the mdx's diaphragm with different ages

• Grant number: 13/06753-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 01, 2013
• End date: January 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Thais Borges Lessa
• Supervisor: Dongsheng Duan
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Missouri, Columbia (UM), United States
• Associated to the scholarship: 12/04506-3 - COMPARATIVE STUDY OF CONTRACTILITY AND PASSIVE PROPERTIES OF THE MDX DIAPHRAGM IN DIFFERENT AGES, BP.DR

Abstract

The diaphragm is the main muscle of breathing that in action with other respiratory muscles performs chest wall expansion and therefore pulmonary ventilation. The Duchenne Muscular Dystrophy (DMD) is a genetic recessive disease characterized by severe and progressive muscle weakness and atrophy. Occurs due a mutation in the gene encoding the 427 kDa protein, called dystrophin and affects 1 in 3,600-6,000 newborn males. The main breathing muscle affected by DMD is the diaphragm and respiratory and/or cardiac failure in these patients, is responsible for 80% of the cause of death. The mdx's diaphragm is compatible to clinical trials and similarly the human DMD, the mdx life expectancy is reduced. Considering the diaphragm weakness and the respiratory and /or cardiac failure responsible for 80% of the cause of death, this study aimed to analyze the fatigue of the diaphragm muscle, along with immunofluorescence (the wheat germ agglutinin -WGA) and hydroxyproline quantification in mdx's with different ages and thus, through this innovative technology, perform a complete muscle morpho-function analysis in this musculature. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HAKIM, CHADY H.; LESSA, THAIS B.; JENKINS, GREGORY J.; YANG, NORA N.; AMBROSIO, CARLOS E.; DUAN, DONGSHENG. An improved method for studying mouse diaphragm function. SCIENTIFIC REPORTS, v. 9, DEC 19 2019. (12/04506-3, 13/06753-0)