Expression of microRNAs in the skeletal muscle plasticity: the possible interrelationships with MuRFs effect in the regenerative process

• Grant number: 12/22488-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: July 01, 2013
• End date: January 31, 2019
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Anselmo Sigari Moriscot
• Grantee: William Jose da Silva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 15/24716-0 - Survey for microRNAs targeting MuRF1 and MuRF2 in skeletal muscle regeneration and mass control, BE.EP.DD

Abstract

Skeletal muscle is highly specialized in the production of force and motion, can adapt to different types of stimuli such as increased demand for mechanical, hormonal status and injury. The injury leads to regenerative response aimed at tissue repair. The regenerative process is extremely important to maintaining the quality of skeletal muscle tissue not only after acute injury but also to deal with micro injuries that occur in daily life activities. Thus the knowledge of the mechanisms cellular / molecular involved in the regenerative process are also important. Studies of another ongoing project in our laboratory show that MuRF1 and MuRF2, has an important role during muscle regeneration (results presented in the introduction), that go beyond their well-known roles as E3 ligases in the process of muscle atrophy . We know that these two MuRFs are strongly induced during regeneration by an unknown mechanism. Accordingly which fits this proposal, we hypothesized that certain comprises microRNAs are capable of inhibiting MuRF1 and MurF2 after injury and that these microRNAs are suppressed, allowing the overexpression of MuRFs. Initially seek in the literature and also in silico microRNAs potentially involved with inhibition of MuRFs. Then will elect two microRNAs potentially inhibiting MuRF1 and MuRF2 in the context of skeletal muscle regeneration. Investigate the expression of these microRNAs in vivo and in vitro, experimentally verifying if MuRFs are effectively targets of these microRNAs. Finally will induce rising levels of these microRNAs in vivo and in vitro in order to understand their impact on the regenerative process. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHRISTOFFOLETE, MARCELO AUGUSTO; SILVA, WILLIAM JOSE; RAMOS, GRACIELLE VIEIRA; BENTO, MIRELLA RIBEIRO; COSTA, MONIQUE OLIVEIRA; RIBEIRO, MIRIAM OLIVEIRA; OKAMOTO, MARISTELA MITIKO; LOHMANN, TANIA HELENA; MACHADO, UBIRATAN FABRES; MUSARO, ANTONIO; et al. Muscle IGF-1-Induced Skeletal Muscle Hypertrophy Evokes Higher Insulin Sensitivity and Carbohydrate Use as Preferential Energy Substrate. BIOMED RESEARCH INTERNATIONAL, 2015. (12/22488-2)

RAMOS, G. V.; CRUZ, A.; SILVA, W. J.; ROZANSKI, A.; BAPTISTA, I. L.; SILVESTRE, J. G.; MORISCOT, A. S.. Thyroid hormone upregulates MDM2 in rat type I fibre: Implications for skeletal muscle mass regulation. ACTA PHYSIOLOGICA, v. 222, n. 4, APR 2018. (12/13071-0, 17/09398-8, 12/13315-7, 12/22488-2, 15/04090-0)

CHRISTOFFOLETE, MARCELO AUGUSTO; SILVA, WILLIAM JOSE; RAMOS, GRACIELLE VIEIRA; BENTO, MIRELLA RIBEIRO; COSTA, MONIQUE OLIVEIRA; RIBEIRO, MIRIAM OLIVEIRA; OKAMOTO, MARISTELA MITIKO; LOHMANN, TANIA HELENA; MACHADO, UBIRATAN FABRES; MUSARO, ANTONIO; et al. Muscle IGF-1-Induced Skeletal Muscle Hypertrophy Evokes Higher Insulin Sensitivity and Carbohydrate Use as Preferential Energy Substrate. BIOMED RESEARCH INTERNATIONAL, v. 2015, p. 8-pg., 2015-01-01. (12/22488-2)

CRUZ, ANDRE; FERIAN, ANDREA; ALVES, PAULA K. N.; SILVA, WILLIAM JOSE; BENTO, MIRELLA RIBEIRO; GASCH, ALEXANDER; LABEIT, SIEGFRIED; MORISCOT, ANSELMO SIGARI. Skeletal Muscle Anti-Atrophic Effects of Leucine Involve Myostatin Inhibition. DNA AND CELL BIOLOGY, v. 39, n. 12, NOV 2020. (12/22488-2, 13/19387-2, 15/04090-0, 17/09398-8, 18/24419-4)

SILVA, WILLIAM JOSE; BAPTISTA, IGOR LUCHINI; SILVESTRE, JOAO GUILHERME; YAN, CHAO IRENE; MORISCOT, ANSELMO SIGARI. Survey on microRNAs Targeting MuRF1 and MuRF2 in Regenerating Skeletal Muscle. FASEB JOURNAL, v. 30, p. 2-pg., 2016-04-01. (12/22488-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, William Jose da. MicroRNAs in muscle plasticity: overexpression of miR-29c in modulation of skeletal muscle mass.. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.