The role of the magnitude of muscle damage in hypertrophic responses

• Grant number: 12/24499-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2013
• End date: April 30, 2017
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Carlos Ugrinowitsch
• Grantee: Felipe Romano Damas Nogueira
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 14/19594-0 - The role of muscle damage in muscle protein synthesis modulation, BE.EP.DR

Abstract

It is not yet established whether muscle damage plays an important role in the chronic hypertrophic process in response to strength training in humans, since the magnitude of muscle damage is significantly attenuated throughout training sessions. Therefore, the aim of this study is investigate if the attenuation of muscle damage during training sessions reduces the rate of myofibrillar protein synthesis and activation of the intracellular mechanism of protein synthesis and, consequently, the magnitude of the hypertrophic response during 12 weeks of strength training in young men. It will be realized functional evaluations and muscle biopsies (before and immediately after the first, fifth and last training sessions) that will allow the analysis of the different magnitudes of muscle damage and their associated hypertrophic responses. The extent of damage will be assessed by quantification of maximum torque, sarcomeral Z-line integrity, disrupted desmin, ultrasound echo intensity. The rate of myofibrillar protein synthesis will be quantified through oral ingestion of deuterated water and muscle biopsies and the activation of hypertrophic mechanisms will be evaluated through the activation, proliferation and differentiation of satellite cells and myonuclei incorporation, and the responses of interleukin 15, fibroblast growth factor and growth factor induced by mechanical stress. The increase in muscle cross-sectional area will be evaluated both in whole muscle by the reconstruction technique of ultrasound images and in isolated fibers by histochemical analysis. Our hypothesis is that greater magnitudes of muscle damage (which will occur at the beginning of the training period) will induce a higher rate of myofibrillar protein synthesis and activation of the hypertrophic mechanism and with the course of training, the magnitude of damage will progressively be attenuated and the hypertrophic responses will also gradually decrease. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DAMAS, FELIPE; PHILLIPS, STUART M.; LIBARDI, CLEITON A.; VECHIN, FELIPE C.; LIXANDRAO, MANOEL E.; JANNIG, PAULO R.; COSTA, LUIZ A. R.; BACURAU, ALINE V.; SNIJDERS, TIM; PARISE, GIANNI; et al. Resistance training-induced changes in integrated myofibrillar protein synthesis are related to hypertrophy only after attenuation of muscle damage. JOURNAL OF PHYSIOLOGY-LONDON, v. 594, n. 18, p. 5209-5222, SEP 15 2016. (14/19594-0, 12/24499-1, 13/21218-4)

DAMAS, F.; NOSAKA, K.; LIBARDI, C. A.; CHEN, T. C.; UGRINOWITSCH, C.. Susceptibility to Exercise-Induced Muscle Damage: a Cluster Analysis with a Large Sample. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 37, n. 8, p. 633-640, JUL 2016. (14/19594-0, 12/24499-1, 13/21218-4)

FOCHI, A. G.; DAMAS, F.; BERTON, R.; ALVAREZ, I; MIQUELINI, M.; SALVINI, T. F.; LIBARDI, C. A.. Greater eccentric exercise-induced muscle damage by large versus small range of motion with the same end-point. BIOLOGY OF SPORT, v. 33, n. 3, p. 285-289, 2016. (12/24499-1, 11/22122-5, 14/19594-0, 13/21218-4)

DAMAS, FELIPE; UGRINOWITSCH, CARLOS; LIBARDI, CLEITON A.; JANNIG, PAULO R.; HECTOR, AMY J.; MCGLORY, CHRIS; LIXANDRAO, MANOEL E.; VECHIN, FELIPE C.; MONTENEGRO, HORACIO; TRICOLI, VALMOR; et al. Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 118, n. 12, p. 2607-2616, DEC 2018. (14/19594-0, 16/24259-1, 17/04299-1, 12/24499-1, 13/21218-4, 18/13064-0)

DAMAS, F.; LIBARDI, C. A.; UGRINOWITSCH, C.; VECHIN, F. C.; LIXANDRAO, M. E.; SNIJDERS, T.. Early- and later-phases satellite cell responses and myonuclear content with resistance training in young men (vol 13, e0191039, 2018). PLoS One, v. 13, n. 2, p. 1-pg., 2018-02-14. (16/24259-1, 13/21218-4, 12/24499-1, 14/19594-0)

DAMAS, FELIPE; LIBARDI, CLEITON A.; UGRINOWITSCH, CARLOS. The development of skeletal muscle hypertrophy through resistance training: the role of muscle damage and muscle protein synthesis. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 118, n. 3, p. 485-500, MAR 2018. (14/19594-0, 16/24259-1, 17/04299-1, 12/24499-1, 13/21218-4)

DAMAS, FELIPE; LIBARDI, CLEITON A.; UGRINOWITSCH, CARLOS; VECHIN, FELIPE C.; LIXANDRAO, MANOEL E.; SNIJDERS, TIM; NEDERVEEN, JOSHUA P.; BACURAU, ALINE V.; BRUM, PATRICIA; TRICOLI, VALMOR; et al. Early- and later-phases satellite cell responses and myonuclear content with resistance training in young men. PLoS One, v. 13, n. 1, JAN 11 2018. (14/19594-0, 16/24259-1, 12/24499-1, 13/21218-4)

DAMAS, FELIPE; PHILLIPS, STUART; VECHIN, FELIPE CASSARO; UGRINOWITSCH, CARLOS. A Review of Resistance Training-Induced Changes in Skeletal Muscle Protein Synthesis and Their Contribution to Hypertrophy. SPORTS MEDICINE, v. 45, n. 6, p. 801-807, JUN 2015. (12/24499-1)

DAMAS, FELIPE; PHILLIPS, STUART M.; LIXANDRAO, MANOEL E.; VECHIN, FELIPE C.; LIBARDI, CLEITON A.; ROSCHEL, HAMILTON; TRICOLI, VALMOR; UGRINOWITSCH, CARLOS. Early resistance training-induced increases in muscle cross-sectional area are concomitant with edema-induced muscle swelling. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. 116, n. 1, p. 49-56, JAN 2016. (14/19594-0, 12/24499-1, 13/21218-4)

DAMAS, FELIPE; PHILLIPS, STUART M.; LIBARDI, CLEITON A.; VECHIN, FELIPE C.; LIXANDRAO, MANOEL E.; JANNIG, PAULO R.; ROSCHEL, HAMILTON; COSTA, LUIZ A. R.; TRICOLI, VALMOR; UGRINOWITSCH, CARLOS. Muscle Damage Over A Resistance-training Period: Repeated Bout Effect Analysed Through Direct And Indirect Markers. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 48, n. 5, p. 1-pg., 2016-05-01. (12/24499-1, 13/21218-4, 14/19594-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOGUEIRA, Felipe Romano Damas. Does muscle damage promote hypertrophy? The fall of a paradigm supported by integrated muscle protein synthesis analysis. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Educação Física e Esportes (EEFE/BT) São Paulo.