Effect of Tyrosol on virulence factors of Candida albicans, Candida glabrata and Streptococcus mutans

• Grant number: 13/03273-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2013
• End date: June 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Alberto Carlos Botazzo Delbem
• Grantee: Douglas Roberto Monteiro
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The present study aims to evaluate the effect of different Tyrosol concentrations on the hydrolytic enzymes production by Candida albicans and Candida glabrata, on the lactic acid production by Streptococcus mutans and on the ability to form single and mixed biofilms of the three species mentioned. First, according to the microdilution method, it will determined the minimum inhibitory concentration of Tyrosol capable of inhibiting the growth of each strain in the planktonic state. After, single and mixed biofilms of the three species will be formed (for 48 hours) in the presence of different Tyrosol concentrations on specimens of acrylic resin and hydroxyapatite within 24-wells microtiter plates. Subsequently, the enzymatic activity (proteinase, phospholipase and hemolytic) of Candida species will be evaluated using specific culture medium for each type of hydrolytic enzyme, while the lactic acid production by S. mutans will be measured by the Clinpro Cario L-Pop Kit. The effect of Tyrosol on the biofilm formation ability will be quantified by counting the number of culturable cells, total biomass (crystal violet staining assay) and metabolic activity of the cells. Moreover, the effect of Tyrosol on preformed biofilms will be assessed through the previously mentioned assays. Biofilm structure after treatment with Tyrosol will be visualized by scanning electron microscopy. Controls devoid Tyrosol will be included in all analyzes, and all tests will be performed in triplicate on three different occasions. Data will be analyzed to employ the most appropriate statistical test. P < .05 will be considered statistically significant.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO VALE, L. R.; DELBEM, A. C. B.; ARIAS, L. S.; FERNANDES, R. A.; VIEIRA, A. P. M.; BARBOSA, D. B.; MONTEIRO, D. R.. Differential effects of the combination of tyrosol with chlorhexidine gluconate on oral biofilms. ORAL DISEASES, v. 23, n. 4, p. 537-541, MAY 2017. (13/10285-2, 13/03273-8)

MONTEIRO, DOUGLAS ROBERTO; ARIAS, LAIS SALOMAO; FERNANDES, RENAN APARECIDO; STRAIOTO, FABIANA GOUVEIA; BARBOSA, DEBORA BARROS; PESSAN, JULIANO PELIM; BOTAZZO DELBEM, ALBERTO CARLOS. Role of tyrosol on Candida albicans, Candida glabrata and Streptococcus mutans biofilms developed on different surfaces. AMERICAN JOURNAL OF DENTISTRY, v. 30, n. 1, p. 35-39, FEB 2017. (13/10285-2, 14/05507-9, 13/03273-8)

ARIAS, L. S.; DELBEM, A. C. B.; FERNANDES, R. A.; BARBOSA, D. B.; MONTEIRO, D. R.. Activity of tyrosol against single and mixed-species oral biofilms. Journal of Applied Microbiology, v. 120, n. 5, p. 1240-1249, MAY 2016. (13/10285-2, 14/05507-9, 13/03273-8)