Evaluation of the role of cardiac SERCA2a in rats submitted to supravalvar aortic stenosis with heart disfunction

• Grant number: 13/09830-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2013
• End date: August 31, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Antonio Carlos Cicogna
• Grantee: Izabelle Ferreira da Silva Mazeto
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In conditions of pressure overload, the heart undergoes an adaptive process of cardiac remodeling (CR). Among the ways this modification presents itself, ventricular hypertrophy stands out. It initially determines an improvement in systolic function, seeking to maintain heart stability. However in the long term the CR represents a risk factor for ventricular dysfunction and heart failure. Among the available experimental models used to study the effects of pressure overload, there is the Supravalvar Aortic Stenosis (SVAoS) which, due to its progressive characteristics, resembles the most the equivalent condition in the human being. In this model, several authors have detected diastolic dysfunction in rats 6 weeks after the surgery. Other researchers have also found a reduction in the expression of SERCA2a. However, studies, including one in our laboratory, have found no objective relation between the decrease of SERCA2a and the reduced diastolic performance in rats with 6 weeks of aortic stenosis. Therefore, this project's goal is: 1) to evaluate the presence of heart dysfunction on the left ventricle and myocardium 18 weeks after SVAoS induction in rats, using echocardiogram e isolated papillary muscle, respectively; 2) to analyze the participation of SERCA2a in the process of heart dysfunction, by blocking this protein on the isolated papillary muscle. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAZETO, I. F. S.; OKOSHI, K.; SILVEIRA, C. F. S. M. P.; SANT'ANA, P. G.; DA SILVA, V. L.; MOTA, G. A. F.; DE SOUZA, S. L. B.; VILEIGAS, D. F.; PADOVANI, C. R.; CICOGNA, A. C.. Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload. Brazilian Journal of Medical and Biological Research, v. 54, n. 4, 2021. (11/21366-8, 13/09830-6)