|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||August 01, 2013|
|Effective date (End):||July 31, 2014|
|Field of knowledge:||Biological Sciences - Genetics - Mutagenesis|
|Principal Investigator:||Daisy Maria Favero Salvadori|
|Grantee:||André Luiz Ventura Sávio|
|Home Institution:||Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil|
Bladder cancer is one of the tumors with the highest cost for health systems due to the high recurrence rates and need for routine cytology (cystoscopy). The chemotherapeutic protocols for bladder cancer include the drugs methotrexate, vinblastine, cisplatin and doxurubicina (MVAC protocol), or the combination of gemcitabine and cisplatin. In most cases, these protocols act by inducing DNA damage and molecular events that result in changes in the cell cycle, DNA repair and apoptosis. The high toxicity of these protocols has stimulated studies on alternative treatments, especially for identifying natural compounds with antineoplastic potential. Allyl isothiocyanate (AITC), found in the seed of mustard and cruciferous vegetables, has attracted attention, since it has been demonstrated its effect on cell cycle and apoptosis in tumor cells, including those from prostate and colon cancer. Its high bioavailability and rapid absorption in bladder make the AITC a promising agent for treatment of urothelial cancer. However, data in literature differ about the possible mechanisms by which AITC might exert its action. Previous studies conducted in our laboratory shown that the AITC has genotoxic effect in different bladder tumor cell lines, inducing G2 cell cycle arrest and apoptosis. Continuing the research on possible AITC anticancer potential, the present study aims to evaluate protein profiling (by mass spectrometry) and the expression of genes related to apoptosis (Bcl-2 and Bax), cell cycle (CDK-1) and DNA repair system (MSH2, CDK7, CCNH, NTHL1, OGG1, RAD51L1 etc), in wild-type (RT4) and mutated (T24) bladder tumor cell lines. The results might provide insights about new anticancer compound.