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Gene and protein expressions in bladder cancer cell lines treated with allyl isothiocyanate (mustard oil)

Grant number: 13/04511-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2013
Effective date (End): July 31, 2014
Field of knowledge:Biological Sciences - Genetics - Mutagenesis
Principal Investigator:Daisy Maria Favero Salvadori
Grantee:André Luiz Ventura Sávio
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Bladder cancer is one of the tumors with the highest cost for health systems due to the high recurrence rates and need for routine cytology (cystoscopy). The chemotherapeutic protocols for bladder cancer include the drugs methotrexate, vinblastine, cisplatin and doxurubicina (MVAC protocol), or the combination of gemcitabine and cisplatin. In most cases, these protocols act by inducing DNA damage and molecular events that result in changes in the cell cycle, DNA repair and apoptosis. The high toxicity of these protocols has stimulated studies on alternative treatments, especially for identifying natural compounds with antineoplastic potential. Allyl isothiocyanate (AITC), found in the seed of mustard and cruciferous vegetables, has attracted attention, since it has been demonstrated its effect on cell cycle and apoptosis in tumor cells, including those from prostate and colon cancer. Its high bioavailability and rapid absorption in bladder make the AITC a promising agent for treatment of urothelial cancer. However, data in literature differ about the possible mechanisms by which AITC might exert its action. Previous studies conducted in our laboratory shown that the AITC has genotoxic effect in different bladder tumor cell lines, inducing G2 cell cycle arrest and apoptosis. Continuing the research on possible AITC anticancer potential, the present study aims to evaluate protein profiling (by mass spectrometry) and the expression of genes related to apoptosis (Bcl-2 and Bax), cell cycle (CDK-1) and DNA repair system (MSH2, CDK7, CCNH, NTHL1, OGG1, RAD51L1 etc), in wild-type (RT4) and mutated (T24) bladder tumor cell lines. The results might provide insights about new anticancer compound.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SÁVIO, André Luiz Ventura. Efeitos toxicogenéticos e toxicogenômicos do Isotiocianato de Alila (óleo de mostarda) em linhagens celulares de carcinoma de bexiga. 2014. 93 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina..

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