The microRNA use for the treatment of prostate cancer: studies in vitro and in vivo

• Grant number: 13/07350-7
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: August 01, 2013
• End date: January 31, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Kátia Ramos Moreira Leite
• Grantee: Alexandre Iscaife
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

MicroRNA (miRNA) are important regulators of gene expression and its amendments are related to the development and progression of cancer. Studies of literature and previous results obtained by our laboratory imply some miRNAs in the progression and biochemical recurrence of prostate cancer. The purpose of this study is to research the role of miRNA in the treatment of metastatic prostate cancer models in vitro and in vivo. Method: The lines PC3, DU145 and LNCaP will be used in in vitro experiments. The miRNA and its antiMiRs will be transfected using the lipofectamine. The rate of proliferation and apoptosis will be evaluate by flow cytometry ploidy. Changes in cellular invasiveness will be analyzed using matrigel. Expression levels of target genes RAS, MYC, MMP9, and CD44 SMARCA5 will be evaluated by the technique of quantitative PCR in real time. Athymic mice, males, 5 or 6 weeks will receive intraventricularly injected with strain PC-3M-Luc-C6. On the 20th will be assessed the development of bone metastases with imaging system IVIS in vivo. The animals will be treated with the miRNA and its antiMiR in 23 and 26 and the lesions will be assessed by measuring with the same equipment on days 30, 34, 38, 42 and 44. On day 48 after the last imaging test animals will be sacrificed and their organs analyzed macroscopically and microscopically. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ISCAIFE, ALEXANDRE; REIS, SABRINA THALITA; MORAIS, DENIS REIS; VIANA, NAYARA IZABEL; DA SILVA, IRAN AMORIM; PIMENTA, RUAN; BORDINI, ANDRE; DIP, NELSON; SROUGI, MIGUEL; MOREIRA LEITE, KATIA RAMOS. Treating metastatic prostate cancer with microRNA-145. Apoptosis, v. 23, n. 7-8, p. 388-395, AUG 2018. (13/07350-7, 12/21966-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ISCAIFE, Alexandre. The use of microRNA for the treatment of prostate cancer: in vitro and in vivo studies. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.