ROLE OF TYPE 3 IODOTHYRONINE DEIODINASE IN THE PILOCARPINE MODEL OF TEMPORAL LOBE EPILEPSY IN MICE

• Grant number: 13/09133-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2013
• End date: June 30, 2015
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Miriam Oliveira Ribeiro
• Grantee: Bruna Pascarelli Pedrico Do Nascimento
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Presbiteriana Mackenzie (UPM). Instituto Presbiteriano Mackenzie. São Paulo , SP, Brazil

Abstract

Epilepsy is a reversible temporary disorder of brain activity characterized by occurrence of spontaneous and recurrent seizures. This disorder is one of the most common neurological conditions in the world wide population. Thyroid hormones (TH) interferes in a myriad of biological process, playing an important role in the development and maintenance of Central Nervous System (CNS). Deiodinases D1, D2 and D3 regulate the metabolism of biologically active triiodothyronine and thus thyroid hormone regulation of the whole organism. D3 deiodinase is the main physiological inactivator of TH, causing local hypothyroidism. In healthy adults D3 levels are very low, but its expression and activity increase significantly in critically ill patients. Respiratory changes frequently occur in seizures, which may lead to increased expression and activity of D3 in some brain regions. Thus, our hypothesis contemplates the possibility of hypoxia from epilepsy leads to increased expression and activity of D3 and that this mechanism represents a protective element to the nervous tissue. Therefore, in order to determine the role of D3 in brain tissue, D3 knockout mice (HtzKOD3) will be subjected to pilocarpine model of epilepsy and then D3, HIF-1±, and neuroglobine expression will be analyzed by immunohistochemistry (IHC), as well as the T3-regulated genes expression in the brain by real-time PCR and the proteins expression related to the activity of TH in the brain by Western Blotting.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO, BRUNA P. P.; BOCCO, BARBARA M. L. C.; FERNANDES, GUSTAVO W.; FONSECA, TATIANA L.; MCANINCH, ELIZABETH A.; CARDOSO, CAROLINA V.; BONDAN, EDUARDO F.; NASSIF, RENATA J.; CYSNEIROS, ROBERTA M.; BIANCO, ANTONIO C.; et al. Induction of Type 2 Iodothyronine Deiodinase After Status Epilepticus Modifies Hippocampal Gene Expression in Male Mice. Endocrinology, v. 159, n. 8, p. 3090-3104, AUG 2018. (13/09133-3, 15/13235-1)