Evaluation of cryopreservation methods for preservation of Haemonchus contortus in laborator

• Grant number: 13/13360-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Estevam Guilherme Lux Hoppe
• Grantee: Alan Jonathan Pereira Testi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Cryopreservation of parasites allows the maintenance of these pathogens in the laboratory at relatively low cost, eliminating the use of animals for this purpose. This study aims to evaluate different cryopreservation techniques, using as variables the freezing weather (slow or fast), using ethylene glycol as criopreservante or absence and desembainhamento or maintenance of the sheath of L3 before freezing. This will used strain of Haemonchus contortus in particular for its ease of obtaining and maintenance in donor animals. How viabilidadem parameters will be evaluated motility and survival rates of all larvae after two months of freezing. The resulting larvae of the protocols considered good shall be tested for infectivity in animals and to determine if, even after the process, are able to establish themselves in the host. It is hoped that this study, to determine an appropriate technique which can be extrapolated to the preservation of nematode parasites of wild animals.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TESTI, Alan Jonathan Pereira. Avaliação das técnicas de criopreservação para manutenção de linhagens de Haemonchus contortus em laboratório. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.