Influence of genotype of cytochrome P450 (CYP2C9) on pharmacokinetics of piroxicam

• Grant number: 13/18454-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2014
• End date: December 31, 2014
• Field of knowledge: Biological Sciences - Pharmacology
• Principal Investigator: Adriana Maria Calvo
• Grantee: Mariel Tavares de Oliveira Prado Bergamo
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Pharmacogenetics is an area of Pharmacology that studies the contribution of genetic factors to individual responses to drugs. This branch of science involves the variability in pharmacodynamics and pharmacokinetics through the study of polymorphisms in genes encoding receptors, as well as in drug metabolism, where this area of Pharmacology has been growing and achievinging its first results with clinical use. The NSAIDS are metabolized by cytochrome P450 (CYP) family, predominantly CYP2C9. The goal of this study is to evaluate the different gene haplotypes for the pharmacokinetics of piroxicam between the different gene haplotypes for CYP2C9 that are found in this population. Therefore, 10 volunteers will be genotyped and fenotyped for this gene and their data will be confronted with the data found in the Brazilian population. For the analysis of the proposed gene, saliva will be collected and serve as a source of genomic DNA. For the molecular analysis, polymerase chain reaction (PCR) with tests validated and produced by Applied Biosystems® will be performed. For the pharmacokinetics, saliva and blood samples will be collected at various times according to protocols available in the literature, and piroxicam concentrations in the samples will be measured by HPLC. The analysis of the results will be described with a significance level of 0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALVO, ADRIANA MARIA; DE OLIVEIRA PRADO, MARIEL TAVARES; DIONISIO, THIAGO JOSE; MARQUES, MARIA PAULA; BROZOSKI, DANIEL THOMAS; LANCHOTE, VERA LUCIA; CARDOSO FARIA, FLAVIO AUGUSTO; SANTOS, CARLOS FERREIRA. Effective method for the detection of piroxicam in human plasma using HPLC. Brazilian Oral Research, v. 30, n. 1, 2016. (09/17851-8, 13/18454-8)

CALVO, ADRIANA MARIA; DE OLIVEIRA PRADO, MARIEL TAVARES; DIONISIO, THIAGO JOSE; MARQUES, MARIA PAULA; BROZOSKI, DANIEL THOMAS; LANCHOTE, VERA LUCIA; CARDOSO FARIA, FLAVIO AUGUSTO; SANTOS, CARLOS FERREIRA. Effective method for the detection of piroxicam in human plasma using HPLC. Brazilian Oral Research, v. 30, 2016. (13/18454-8, 09/17851-8)

CALVO, ADRIANA MARIA; DE OLIVEIRA PRADO, MARIEL TAVARES; DIONISIO, THIAGO JOSE; MARQUES, MARIA PAULA; BROZOSKI, DANIEL THOMAS; LANCHOTE, VERA LUCIA; CARDOSO FARIA, FLAVIO AUGUSTO; SANTOS, CARLOS FERREIRA. Effective method for the detection of piroxicam in human plasma using HPLC. BRAZILIAN ORAL RESEARCH, v. 30, n. 1, p. 6-pg., 2016-01-01. (13/18454-8, 09/17851-8)