The phenomenon known as programming suggests that stimuli or aggression during intrauterine life may result in permanent changes in physiology and metabolism of offspring, increasing the risk of diseases in adulthood. Studies have demonstrated that periodontal disease (PD) and adipose tissue augmentation elevate the level of plasmatic cytokines. And cytokines, such as TNF-alpha, have been associated with reduced expression of GLUT4 and insulin resistance. Knowing that GLUT4 is important for glucose homeostasis, the aim of this study is to evaluate: the content of the glucose transporter protein GLUT4 and its translocation to the plasmatic membrane in gastrocnemius skeletal muscle of adult rats, offspring of rats with periodontal disease. The rats will be divided into two groups: 1) with periodontal disease (PED), in which the disease is induced by ligation with silk thread around the 1st molar, 2) control rats (CN). Seven days after ligature placement, the rats of both groups will be placed for mating, verifying daily by vaginal smear, the day of copulation. Pregnant rats will be separated into individual boxes. When male offspring of these rats completed 75 days, will be performing the experiments: 1) evaluation of the content of the glucose transporter protein GLUT4 and its translocation to the plasmatic membrane in gastrocnemius skeletal muscle by the method of "Western Blotting"; 2) plasma concentrations of glucose and insulin.
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