Advanced search
Start date
Betweenand
Related content

EVALUATION OF GLUT4 CONTENT AND ITS TRANSLOCATION TO THE PLASMATIC MEMBRANE IN MUSCLE TISSUE OF ADULT RATS, OFFSPRING OF RATS WITH PERIODONTAL DISEASE

Grant number: 13/08783-4
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2013
Effective date (End): January 31, 2015
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal researcher:Doris Hissako Matsushita
Grantee:Maria Sara de Lima Coutinho Mattera
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The phenomenon known as programming suggests that stimuli or aggression during intrauterine life may result in permanent changes in physiology and metabolism of offspring, increasing the risk of diseases in adulthood. Studies have demonstrated that periodontal disease (PD) and adipose tissue augmentation elevate the level of plasmatic cytokines. And cytokines, such as TNF-alpha, have been associated with reduced expression of GLUT4 and insulin resistance. Knowing that GLUT4 is important for glucose homeostasis, the aim of this study is to evaluate: the content of the glucose transporter protein GLUT4 and its translocation to the plasmatic membrane in gastrocnemius skeletal muscle of adult rats, offspring of rats with periodontal disease. The rats will be divided into two groups: 1) with periodontal disease (PED), in which the disease is induced by ligation with silk thread around the 1st molar, 2) control rats (CN). Seven days after ligature placement, the rats of both groups will be placed for mating, verifying daily by vaginal smear, the day of copulation. Pregnant rats will be separated into individual boxes. When male offspring of these rats completed 75 days, will be performing the experiments: 1) evaluation of the content of the glucose transporter protein GLUT4 and its translocation to the plasmatic membrane in gastrocnemius skeletal muscle by the method of "Western Blotting"; 2) plasma concentrations of glucose and insulin.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE LIMA COUTINHO MATTERA, MARIA SARA; CHIBA, FERNANDO YAMAMOTO; DE OLIVEIRA DA MOTA, MAX SANDER; PEREIRA, RENATO FELIPE; ERVOLINO, EDILSON; CHAVES NETO, ANTONIO HERNANDES; PESCININI SALZEDAS, LEDA MARIA; SCARAMELE, NATALIA FRANCISCO; SILVA, CRISTINA ANTONIALI; OKAMOTO, MARISTELA MITIKO; MACHADO, UBIRATAN FABRES; SUMIDA, DORIS HISSAKO. Maternal periodontitis decreases plasma membrane GLUT4 content in skeletal muscle of adult offspring. Life Sciences, v. 148, p. 194-200, MAR 1 2016. Web of Science Citations: 3.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MATTERA, Maria Sara de Lima Coutinho. Avaliação da fosforilação da AKT e do conteúdo da proteína transportadora de glicose GLUT4 em tecido muscular esquelético de ratos adultos, proles de ratas com doença periodontal. 2015. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Odontologia (Campus de Araçatuba)..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.