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Hematological parameters and serum proteinogram, with emphasis on acute phase proteins and immunoglobulins, in newborn buffalo calves

Grant number: 13/17860-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2013
Effective date (End): October 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:José Jurandir Fagliari
Grantee:Natália Cristina Benincasa
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The present study aims to evaluate the hematological parameters and serum protein concentrations, with an emphasis on acute phase proteins and immunoglobulins in healthy newborn buffalo calves. Venous blood samples of Jaffarabadi healthy male and female buffalo calves with 1 to 7-day-old (G1, n = 20), 8 to 14-day-old (G2, n = 20) 15 to 21-day-old (G3, n = 20), and 22 to 28-day-old (G4, n = 20) will be collected. Blood cell count, white blood cell, platelet count, hemoglobulin concentration, packed cell volume and hematological indices (mean corpuscular volume - MCV, mean corpuscular hemoglobin - MCH and mean corpuscular hemoglobin concentration - MCHC) will be determined. Will also be evaluated serum activity of gamma-glutamyl transferase (GGT) and serum iron. The concentrations of acute phase proteins and serum immunoglobulins will be determined by means of sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE).