Evaluation of microbial interactions and the role of surface adhesins of initial colonizers Streptococcus oralis and Actinomyces naeslundii in the formation and structure Candida albicans biofilms.

• Grant number: 13/15884-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2013
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Altair Antoninha Del Bel Cury
• Grantee: Indira Moraes Gomes Cavalcanti
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The present study aims to investigate the microbial interactions and evaluate the role of surface adhesins of initial colonizers Streptococcus oralis and Actinomyces naeslundii in the formation and structure of Candida albicans biofilms. For this, Poly (methyl-methacrylate)resin discs will be fabricated and standardized according to the surface roughness, simulating the inner face of a prosthesis. The discs will be randomly distributed in a 24-well plate and given 1 ml of stimulated and filtered saliva for salivary film formation. Then, the discs will be transferred to a new 24-well plate in which occurs the formation of biofilms containing microorganisms Actinomyces naeslundii (An), Streptococcus oralis (So) and Candida albicans (Ca), divided into three groups: (I)biofilm containing An and Ca (II) containing So and Ca and(III)the biofilm containing the three species. Biofilms will be evaluated during adhesion (1.5 hours) and at 24 and 48 hours of development. Cell viability of each micro-organism will be determined by quantifying the polymerase chain reaction (qPCR) and by counting the colony forming units (CFU). The bacterial adhesins and protein co-aggregation CSH-like and Ag I/II will be investigated from assays that comprise the genetic modification of microorganisms, construction of mutants microorganisms and determining the presence of the adhesins by molecular assays. The structure and organization of biofilms will be evaluated with confocal laser microscopy and scanning electron microscopy (SEM).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVALCANTI, INDIRA M. G.; NOBBS, ANGELA H.; RICOMINI-FILHO, ANTONIO PEDRO; JENKINSON, HOWARD F.; DEL BEL CURY, ALTAIR A.. Interkingdom cooperation between Candida albicans, Streptococcus oralis and Actinomyces oris modulates early biofilm development on denture material. PATHOGENS AND DISEASE, v. 74, n. 3, APR 2016. (13/15884-1)

CAVALCANTI, I. M. G.; DEL BEL CURY, A. A.; JENKINSON, H. F.; NOBBS, A. H.. Interactions between Streptococcus oralis, Actinomyces oris, and Candida albicans in the development of multispecies oral microbial biofilms on salivary pellicle. Molecular Oral Microbiology, v. 32, n. 1, p. 60-73, FEB 2017. (13/15884-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAVALCANTI, Indira Moraes Gomes. Evaluation of microbial interactions and the role of Streptococcus oralis and Actinomyces oris adhesins in the formation and structure of mixed biofilms with Candida albicans. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.