Characterization of dupA gene of Helicobacter pylori and gene expression analysis of E-Caderin gene in gastric biopses samples from dyspeptic patients and gastric cancer.

• Grant number: 13/21285-3
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2013
• Effective date (End): November 30, 2014
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Lucas Trevizani Rasmussen
• Grantee: Mariane Avante Ferraz
• Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil

Abstract

Duodenal ulcer-promoting gene A (dupA), is a novel virulence factor, in Helicobacter pylori and was associated with duodenal ulcer development and reduced risk for gastric carcinoma in some populations. In additional, E-caderin gene seems to be associated with infiltrative and metastatic ability of gastric tumors. Previous studies suggesting that gene inactivation of E-cadherin by H. pylori may be related to gastric carcinogenesis. Therefore, the aims of this study are: to determine the presence of dupA gene and evaluate association with other virulence factors such as cagA, vacA previous detected, and analysis the gene expression of E-cadherin gene in 110 samples from gastric biopsies of adults patients with peptic symptoms and / or gastric cancer, in order to correlate the expression of E-Caderin gene and dupA virulence factor on the development and in the progression of gastric changes. The development of this approach may open new perspectives for the characterization of factors involved in peptic diseases, gene regulation, as well as to elucidate the interaction among genes involved in gastric diseases, improving the understanding of the pathophysiological mechanisms of these diseases.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZABAGLIA, LUANNA MUNHOZ; FERRAZ, MARIANE AVANTE; PEREIRA, WEENDELLY NAYARA; ORCINI, WILSON APARECIDO; DE LABIO, ROGER WILLIAN; NETO, AGOSTINHO CALEMAN; WISNIESKI, FERNANDA; DE OLIVEIRA, JULIANA GARCIA; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Lack of association among TNF-alpha gene expression,-308 polymorphism (G > A) and virulence markers of Helicobacter pylori. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 21, DEC 30 2015. Web of Science Citations: 4.

LUANNA MUNHOZ ZABAGLIA; MARIANE AVANTE FERRAZ; WEENDELLY NAYARA PEREIRA; WILSON APARECIDO ORCINI; ROGER WILLIAN DE LABIO; AGOSTINHO CALEMAN NETO; FERNANDA WISNIESKI; JULIANA GARCIA DE OLIVEIRA; MARILIA DE ARRUDA CARDOSO SMITH; SPENCER LUIZ MARQUES PAYÃO; LUCAS TREVIZANI RASMUSSEN. Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 21, p. -, 2015.