Bone marrow-derived stem cell in a model of myocardial infarction induced by isoproterenol administrationin in rats

• Grant number: 13/19426-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2013
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Patrícia Fidelis de Oliveira
• Grantee: Mariana Spinardi
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Myocardial infarction (MI) is an important cause of death worldwide, making it necessary to search for therapeutic approaches. Within this context, cell therapy in MI using stem cells (CT) contained in bone marrow mononuclear portion (BMMC) has been investigated. This study aims to: 1) echocardiographic and hemodynamic functional characterization of MI model induced by isoproterenol administration (ISO) in rats; 2) analysis of BMMC migration to the infarcted area and 3) evaluation BMMC administration on cardiac function of infarcted rats. For this purpose, male Wistar rats (200-250g) will receive 0.5 mL of ISO (250 mg/kg in 0.9% NaCl) subcutaneously (n=10) or intraperitoneally (n=10) for 2 consecutive days. Control rats will receive 0.9% NaCl (0.5 mL) by the respective routes of administration. Functional characterization of MI model will be made by echocardiography (1, 3 and 6 weeks after MI) and hemodynamic analysis (6 weeks after MI). Once the model is established, there will be a second protocol in which experimental rats (n = 10), 1 week after MI, will be submitted to iv administration (jugular vein) of 4x106 BMMC or an equal volume (0.2 mL) of culture media (n = 10). To study the migration capacity, 5 animals will be sacrificed 24 hours after iv administration for tissue investigation about BMMC presence in the heart. The other animals will be remain in the study until 6th week after MI for functional assessments. This study will allow the characterization of the MI model in our laboratory as well as provide information about the ability of BMMC migration and possible benefits on cardiac function.