Study of the compatibility of atemoya (Annona squamosa L. x Annona cherimola mill.) grafs in araticum-mirim [Annona emarginata (Schltdl.) H. Rainer "variety Mirim"] and araticum-de-terra-fria [Annona emarginata (Schltdl.) H. Rainer "variety terra-fria"]

• Grant number: 13/22036-7
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: January 15, 2014
• End date: April 14, 2014
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Gisela Ferreira
• Grantee: Daniel Baron
• Supervisor: Ana Pina Sobrino
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Spain
• Associated to the scholarship: 11/00853-8 - Study of the compatibility of atemoya(Annona squamosa L. x Annona cherimoya Mill.) grafs in araticum-mirim [Annona emarginata (Schltdl.) H. Rainer "variedade mirim"] and araticum-de-terra-fria [Annona emarginata(Schltdl.) H. Rainer "variedade terra-fria"], BP.DR

Abstract

Grafting is effectively used for the cultivation of commercial fruit species, since it is necessary to ensure the genetic characteristics of producing species with the use of selected clones. Furthermore, the use of rootstocks resistant to soil pathogens is important when working with species susceptible such atemoya. However, despite that the graft is a common and widespread technique, the mechanisms involved in graft (in-) compatibility of woody trees are not well elucidated. The aim of this study is to determine differentially expressed proteins between combinations scion atemoya (Annona squamosa L. x Annona cherimola Mill.) grafted onto araticum-mirim [A. emarginata (Schltdl.) H. Rainer 'variety mirim'] and araticum-de-terra-fria [A. emarginata (Schltdl.) H. Rainer 'variety terra-fria'], kept in crop protection, regarding the formation of seedlings for field cultivation. The plant material that will be used is from the grafted area at different post-grafting times (0, 15, 30 and 60 days after grafting, DAG). The SDS-PAGE technique combined with MALDI-TOF will be used to quantify protein expression of UDP-glucose pyrophosphorylase (UGP) gene, candidate in studies of compatibility/incompatibility of grafting plants between different combinations. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARON, DANIEL; BRAVO, JULIANA P.; MAIA, IVAN G.; PINA, ANA; FERREIRA, GISELA. UGP gene expression and UDP-glucose pyrophosphorylase enzymatic activity in grafting annonaceous plant. ACTA PHYSIOLOGIAE PLANTARUM, v. 38, n. 3, MAR 2016. (13/22036-7, 11/00853-8)