Impact of androgens on the inflammatory response, osteoclastogenesis and expression of sex hormones receptors on periodontal tissues of female rats under conditions of periodontal health, disease and repair

• Grant number: 13/23116-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2014
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: João Paulo Steffens
• Grantee: Maria Carolina da Costa Albaricci
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The etiopathogenesis of the immune-inflammatory responses associated with periodontal disease is a strictly regulated and complex process that involves many molecules related with different cell signaling pathways. In this context, the interaction of hormones and their receptors, including sex hormones, plays an important role. It is plausible that androgenic hormones role on immune-inflammatory responses is based on a complex interaction network that culminates in host response modulation. Particularly, our group demonstrated that sub- and supraphysiologic androgen levels exacerbate the immune-inflammatory response and periodontal bone loss using a ligature-induced periodontitis model in male rats. Besides, we reported that physiologic levels of testosterone inhibit osteoclastogenesis both directly and indirectly, reducing osteoblast-derived RANKL expression in vitro. Despite the biological plausibility in the participation of androgenic hormones playing an active role on the etiopathogenesis of periodontal diseases, little is known about the importance of androgens on the different events that take place during the progression and reparative phases of periodontal disease in females. This project aims to extend our studies inhibiting androgen and estrogen receptors or administering testosterone with or without aromatase inhibitor in female rats. We will evaluate the histologic profile through Hematoxilina and Eosin (H&E) staining and immunohistochemistry techniques to detect tartrate-resistant acid phosphatase (TRAP), androgen and estrogen receptors in periodontal health, disease and repair.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

STEFFENS, JOAO PAULO; VALENGA, HENRIQUE MEISTER; LEAL SANTANA, LUIS CARLOS; DA COSTA ALBARICCI, MARIA CAROLINA; KANTARCI, ALPDOGAN; SPOLIDORIO, LUIS CARLOS. Role of testosterone and androgen receptor in periodontal disease progression in female rats. Journal of Periodontology, AUG 2019. (13/07833-8, 13/23116-4, 13/12014-6)

STEFFENS, JOAO PAULO; LEAL SANTANA, LUIS CARLOS; PAIVA PITOMBO, JONLENO COUTINHO; RIBEIRO, DANIEL OLIVIO; COSTA ALBARICCI, MARIA CAROLINA; CUBAS WARNAVIN, STEPHANIE VON STEIN; KANTARCI, ALPDOGAN; SPOLIDORIO, LUIS CARLOS. The role of androgens on periodontal repair in female rats. Journal of Periodontology, v. 89, n. 4, p. 486-495, APR 2018. (13/07833-8, 14/02280-3, 13/23116-4, 13/12014-6)

STEFFENS, JOAO PAULO; VALENGA, HENRIQUE MEISTER; LEAL SANTANA, LUIS CARLOS; DA COSTA ALBARICCI, MARIA CAROLINA; KANTARCI, ALPDOGAN; SPOLIDORIO, LUIS CARLOS. Role of testosterone and androgen receptor in periodontal disease progression in female rats. Journal of Periodontology, v. 91, n. 4, p. 9-pg., 2019-08-25. (13/12014-6, 13/23116-4, 13/07833-8)