Chemical-biological prospecting of hydroethanolic extracts of leaves from Astronium urundeuva in the investigation of the antifungal potential

• Grant number: 13/25121-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Taís Maria Bauab
• Grantee: Bruna Vidal Bonifácio
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 15/19011-8 - Astronium urundeuva extract as antifungal agent: exploring the mechanism of action against Candida sp., BE.EP.DR

Abstract

Therapeutic approaches of medicinal plants to control infections caused by pathogenic or opportunistic microorganisms are advantageous because they have potentially reduced side effects and toxicity. Recently, the medicinal use of natural products has been highlighted due to the large and increasing number of strains that have been becoming resistant to conventional antibiotics and can be explained mainly by their indiscriminate use that causes a higher incidence of treatment failures. Therefore, studies involving medicinal plants have gained the focus of researchers who have been searching for natural products in order to combat fungal infections, especially those acquired by Candida species, one of the most frequently involved yeast in fungal infections, for example, vulvovaginal candidiasis. In order to continue previous studies (master) with Astronium urundeuva (Anacardiaceae) leaves' hydroalcoholic extract, which showed a better antifungal activity after being incorporated into the nanostructured system, this project aims to conduct preliminary phytochemical screening and fractionation of A. urundeuva leaves' extract, in vitro and in vivo biological assays against the standard reference strains of C. albicans and C. glabrata, and further proteomic analysis. Therefore, it is expected to discover the main constituent(s) related to the in vitro antifungal activity and subsequently elucidate the mechanism of action of A. urudeuva in this antifungal potential in order to obtain a new therapeutic approach devoted to an in vivo experimental model of vulvovaginal candidiasis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BONIFACIO, BRUNA VIDAL; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SILVEIRA NEGRI, KAMILA MARIA; LOPES, ERICA DE OLIVEIRA; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 5081-5092, 2015. (09/52237-9, 13/25121-5, 13/25432-0)

BONIFACIO, BRUNA VIDAL; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SILVEIRA NEGRI, KAMILA MARIA; LOPES, ERICA DE OLIVEIRA; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 12-pg., 2015-01-01. (09/52237-9, 13/25432-0, 13/25121-5)

BONIFACIO, BRUNA VIDAL; MACHADO VILA, TAISSA VIEIRA; MASIERO, ISADORA FANTACINI; DA SILVA, PATRICIA BENTO; DA SILVA, ISABEL CRISTIANE; LOPES, ERICA DE OLIVEIRA; DOS SANTOS RAMOS, MATHEUS APARECIDO; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; et al. Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata. FRONTIERS IN MICROBIOLOGY, v. 10, NOV 15 2019. (13/25432-0, 09/52237-9, 15/19011-8, 13/25121-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BONIFÁCIO, Bruna Vidal. Chemical-biological prospection of the hydroethanolic extracts of Astronium urundeuva leaves in the investigation of the antifungal potential. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.