Therapeutic approaches of medicinal plants to control infections caused by pathogenic or opportunistic microorganisms are advantageous because they have potentially reduced side effects and toxicity. Recently, the medicinal use of natural products has been highlighted due to the large and increasing number of strains that have been becoming resistant to conventional antibiotics and can be explained mainly by their indiscriminate use that causes a higher incidence of treatment failures. Therefore, studies involving medicinal plants have gained the focus of researchers who have been searching for natural products in order to combat fungal infections, especially those acquired by Candida species, one of the most frequently involved yeast in fungal infections, for example, vulvovaginal candidiasis. In order to continue previous studies (master) with Astronium urundeuva (Anacardiaceae) leaves' hydroalcoholic extract, which showed a better antifungal activity after being incorporated into the nanostructured system, this project aims to conduct preliminary phytochemical screening and fractionation of A. urundeuva leaves' extract, in vitro and in vivo biological assays against the standard reference strains of C. albicans and C. glabrata, and further proteomic analysis. Therefore, it is expected to discover the main constituent(s) related to the in vitro antifungal activity and subsequently elucidate the mechanism of action of A. urudeuva in this antifungal potential in order to obtain a new therapeutic approach devoted to an in vivo experimental model of vulvovaginal candidiasis.
News published in Agência FAPESP Newsletter about the scholarship: