Assessment of the Akt phosphorylation status and the GLUT4 expression in muscle tissue of rats treated with NaF

• Grant number: 13/19586-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2014
• End date: June 30, 2017
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Fernando Yamamoto Chiba
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Over the last years, there has been a significant reduction in the incidence of dental caries in several regions of the world. This has been attributed to the use of fluoridated products, such as toothpaste. Simultaneously, there has been an increase in the prevalence of dental fluorosis. NaF causes glycolysis inhibition, decrease on insulin secretion and hyperglycemia. These responses suggest that NaF can cause insulin resistance. Knowing that F can interfere with carbohydrate metabolism, we felt it was important and fundamental to undertake a study to examine the chronic effect of NaF on: 1) Akt serine and treonine phosphorylation; 2) GLUT4 expression and its rate of translocation to the plasma membrane in skeletal muscle gastrocnemius. For this study, castrated Wistar male rats (1 month of age) will be used. Thirty days after castration, the animals will be randomly divided in two groups: 1) control group (CN) which was subjected to treatment without NaF, but with a solution of NaCl (9.54 mg / kg bw) which contains the same amount of sodium in relation to the group NaF; 2) group NaF (FN) that will be submitted to treatment with NaF administered in the drinking water and F contained in food pellets (F total inferred: 4.0 mg F / Kg bw / day in the form of NaF) during 42 days. After 6 weeks, will be performed : 1 ) assessment of the Akt phosphorylatioGLUT4 expression and its rate of translocation to the plasma membrane in skeletal muscle gastrocnemius.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHIBA, FERNANDO Y.; GARBIN, CLEA A. S.; MATTERA, MARIA S. L. C.; MOTA, MAX S. O.; PEREIRA, RENATO F.; SUMIDA, DORIS H.. CHRONIC TREATMENT WITH A MILD DOSE OF NaF PROMOTES DYSLIPIDEMIA IN RATS. FLUORIDE, v. 48, n. 3, p. 205-212, JUL-SEP 2015. (10/10664-5, 13/19586-5)

CHIBA, FERNANDO YAMAMOTO; SAORI TSOSURA, THAIS VERONICA; PEREIRA, RENATO FELIPE; DE LIMA COUTINHO MATTERA, MARIA SARA; DOS SANTOS, RODRIGO MARTINS; MARANI, FERNANDO; SALIBA GARBIN, CLEA ADAS; SALIBA MOIMAZ, SUZELY ADAS; SUMIDA, DORIS HISSAKO. MILD CHRONIC NaF INTAKE PROMOTES INSULIN RESISTANCE AND INCREASE IN INFLAMMATORY SIGNALING IN THE WHITE ADIPOSE TISSUE OF RATS. FLUORIDE, v. 52, n. 1, p. 18-28, JAN 2019. (13/19586-5)

CHIBA, FERNANDO YAMAMOTO; TSOSURA, THAIS VERONICA SAORI; DE LIMA COUTINHO MATTERA, MARIA SARA; PEREIRA, RENATO FELIPE; DOS SANTOS, RODRIGO MARTINS; FUJII DE OLIVEIRA, RENAN AKIRA; SALIBA GARBIN, CLEA ADAS; BELARDI, BIANCA ELVIRA; PENHA DE OLIVEIRA, SANDRA HELENA; MATSUSHITA, DORIS HISSAKO. CHRONIC SODIUM FLUORIDE INTAKE PROMOTES CHANGES IN INSULIN SIGNALING AND INFLAMMATORY PATHWAYS IN THE SKELETAL MUSCLE OF RATS. FLUORIDE, v. 54, n. 4, p. 14-pg., 2021-10-01. (13/19586-5)