Ruthenium(II) complexes coordinated to dipyridophenazine and dipyridoquinoxaline ligands: cellular assays in tumor cell lines and study of the mechanism of action for their application in anticancer therapies

• Grant number: 13/21611-8
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): March 01, 2014
• Effective date (End): August 03, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal Investigator: Alzir Azevedo Batista
• Grantee: João Paulo Barolli Reis
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

This project aims at the synthesis of ruthenium(II) complexes coordinated to the ligands dppf (1,1'-diphenylphosphinoferrocene), dpq (dipyrido[3,2-f: 2',3'-h]quinoxaline), dppz (dipyrido[3,2-a:2 ', 3'-c]phenazine) dpqQX (dipyrido[3,2-f 2', 3'-h]quinoxaline[2,3-b]quinoxaline), dppzBTDZ (dipyrido[3,2-a: 2 ',3'-c]phenazine-10,11-(2,1,3-thiadiazole) and CO (carbonyl) and to study its physicochemical properties by 1H, 13C{1H}, 31P{1H} NMR spectrometry, mass spectrometry , IR, UV-Vis, cyclic voltammetry, differential pulse and X-ray diffraction. We also intend to perform new complexes in vitro cytotoxicity assays on cell lines derived from human tumors of breast cancer, for example: MDA-MB-231 and MCF7, as well as studies of new complexes interact with potential targets biological, e.g., DNA and macromolecules such as BSA, which will help us in understanding the stability / reactivity thereof in biological systems its possible mechanisms of action. The interactions of the new ruthenium complexes with biomolecules will be evaluated by viscosity assays, atomic force microscopy and spectrophotometric and fluorescence titrations. Cytotoxicity assays will be performed in tumor cell lines MDA-MB-231 and MCF7 (breast cancer) and normal L929 (fibroblasts derived from mice).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BAROLLI, JOAO P.; MAIA, PEDRO I. S.; COLINA-VEGAS, LEGNA; MOREIRA, JANE; PLUTIN, ANA M.; MOCELO, RAUL; DEFLON, VICTOR M.; COMINETTI, MARCIA R.; CAMARGO-MATHIAS, MARIA I.; BATISTA, ALZIR A.. Heteroleptic tris-chelate ruthenium(II) complexes of N,N-disubstituted-N `-acylthioureas: Synthesis, structural studies, cytotoxic activity and confocal microscopy studies. Polyhedron, v. 126, p. 33-41, APR 18 2017. (09/54011-8, 11/21033-9, 11/16380-1, 13/21611-8)

BAROLLI, JOAO P.; CORREA, RODRIGO S.; MIRANDA, FABIO S.; RIBEIRO, JULIANA U.; BLOCH, JR., CARLOS; ELLENA, JAVIER; MORENO, VIRTUDES; COMINETTI, MARCIA R.; BATISTA, ALZIR A.. Polypyridyl Ruthenium Complexes: Novel DNA-Intercalating Agents against Human Breast Tumor. Journal of the Brazilian Chemical Society, v. 28, n. 10, p. 1879-1889, OCT 2017. (13/21611-8, 12/06013-4)