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Increasing scale of anaerobic fluidized bed reactor for removal of anionic and nonionic surfactants in commercial laundry wastewater

Grant number: 13/19025-3
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2014
Effective date (End): August 31, 2018
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Principal Investigator:Maria Bernadete Amâncio Varesche
Grantee:Fabricio Motteran
Home Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery, AP.TEM
Associated scholarship(s):16/06338-1 - Mass spectrometry analysis of by-products and intermediary metabolites from Linear Alkylbenzene Sulfonate under anaerobic degradation, BE.EP.PD


This study will be addressed anaerobic degradation of anionic and nonionic surfactants in commercial laundry wastewater, respectively, linear alkylbenzene sulfonate (LAS) and linear alcohol ethoxylate (LAE), both widely used in the manufacture of detergents and present in sewage and water industrial wastewater. In a certain way they may negatively affect the process of wastewater treatment, either with foam formation or with inhibition of organisms responsible for biological treatment through by-products generated from the surfactant`s degradation. An anaerobic fluidized bed reactor in pilot scale will be used filled with sand as support material. Previously the reactor operation, test of methanogenic potential, toxicicity and sludge choose with better removal efficiency of surfactants, will be performed in batch. The anaerobic fluidized bed reactor in pilot scale will be inoculated with sludge from UASB reactors containing high diversity of microorganisms. The reactor will be operated in 5 stages being (inoculation, adaptation, and stages with commercial laundry wastewater). Physicochemical analyzes of pH, alkalinity, COD, determination of LAS and LAE (through mass spectrometry LC/MS) and volatile organic acids (HPLC) for monitoring the influent and effluent of the reactor will be performed. For the phylogenetic characterization, pyrosequencing analysis of V3 region of 16S RNAr in each reactor operation stage will be carried out. In biological treatment processes the reactor's scale has fundamental importance. The difficulty in scaling up biotechnological processes is the maintenance of stability operating conditions, especially in anaerobic fluidized bed reactor, keep the effective mass transfer, fluidize the bed and hold the biomass attached to the support material. Currently, studies with removal and degradation of surfactants in anaerobic fluidized bed reactor on a bench scale are well consolidated. Thus, this study can fill gaps with regard to anaerobic degradation of anionic and nonionic surfactants present in real wastewater in a fluidized bed reactor in pilot scale as well as evaluating both, by-products generated through the degradation of these compounds and the microbial diversity present in this reactor model.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOTTERAN, FABRICIO; NASCIMENTO, ROSEMEIRE FAGUNDES; NADAI, BIANCA MARQUES; TITATO, GUILHERME MIOLA; DOS SANTOS NETO, ALVARO JOSE; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Identification of Anionic and Nonionic Surfactant and Recalcitrants Compounds in Commercial Laundry Wastewater by GC-MS Analysis After Anaerobic Fluidized Bed Reactor Treatment. WATER AIR AND SOIL POLLUTION, v. 230, n. 12 DEC 2019. Web of Science Citations: 0.
MOTTERAN, FABRICIO; NADAI, BIANCA MARQUES; BRAGA, JULIANA KAWANISHI; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Metabolic routes involved in the removal of linear alkylbenzene sulfonate (LAS) employing linear alcohol ethoxylated and ethanol as co-substrates in enlarged scale fluidized bed reactor. Science of The Total Environment, v. 640, p. 1411-1423, NOV 1 2018. Web of Science Citations: 3.
MOTTERAN, FABRICIO; LIMA GOMES, PAULO C. F.; SILVA, EDSON L.; VARESCHE, MARIA BERNADETE A. Simultaneous determination of anionic and nonionic surfactants in commercial laundry wastewater and anaerobic fluidized bed reactor effluent by online column-switching liquid chromatography/tandem mass spectrometry. Science of The Total Environment, v. 580, p. 1120-1128, FEB 15 2017. Web of Science Citations: 5.
MOTTERAN, FABRICIO; BRAGA, JULIANA K.; SILVA, EDSON L.; VARESCHE, MARIA BERNADETE A. Kinetics of methane production and biodegradation of linear alkylbenzene sulfonate from laundry wastewater. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, v. 51, n. 14, p. 1288-1302, 2016. Web of Science Citations: 6.

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