Evaluation of Biodentine in the formation of hard tissue bridge and biological compatibility of rats pulp

• Grant number: 14/03134-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2014
• End date: August 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Eloi Dezan Junior
• Grantee: Vitor Correa Homse
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study is to evaluate the biocompatibility and capacity of hard tissue bridge formation of endodontic materials Biodentine TM and White MTA Angelus® compared to calcium hydroxide. Pulpotomy will be performed in 60 Wistar rats. The first right and left upper molars will have their coronal pulp exposed and then removed, protecting radicular pulp with the materials to be tested and sealing with glass ionomer, and a group will be directly sealed with glass ionomer as control group. After periods of 7, 15 and 30 days, the jaws will be removed and fixed in 10% formalin followed by demineralization in 10% EDTA and embedded in paraffin. The staining will be held in HE, Brown and Brenn. Microtomography of the parts will be performed prior to demineralization to see whether there was the formation of dentine bridge. The histological evaluation criteria is the hard tissue formation (continuity, thickness and morphology), pulp inflammation (type, severity and extent), and other histological features of the pulp tissue, including the layer of odontoblastic cells and bacterial penetration. The results will be analyzed by Kruskal Wallis (±=0,05).